1t4n: Difference between revisions
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New page: left|200px<br /><applet load="1t4n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t4n" /> '''Solution structure of Rnt1p dsRBD'''<br /> ... |
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[[Image:1t4n.gif|left|200px]]<br /><applet load="1t4n" size=" | [[Image:1t4n.gif|left|200px]]<br /><applet load="1t4n" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1t4n" /> | caption="1t4n" /> | ||
'''Solution structure of Rnt1p dsRBD'''<br /> | '''Solution structure of Rnt1p dsRBD'''<br /> | ||
==Overview== | ==Overview== | ||
Rnt1 endoribonuclease, the yeast homolog of RNAse III, plays an important | Rnt1 endoribonuclease, the yeast homolog of RNAse III, plays an important role in the maturation of a diverse set of RNAs. The enzymatic activity requires a conserved catalytic domain, while RNA binding requires the double-stranded RNA-binding domain (dsRBD) at the C-terminus of the protein. While bacterial RNAse III enzymes cleave double-stranded RNA, Rnt1p specifically cleaves RNAs that possess short irregular stem-loops containing 12-14 base pairs interrupted by internal loops and bulges and capped by conserved AGNN tetraloops. Consistent with this substrate specificity, the isolated Rnt1p dsRBD and the 30-40 amino acids that follow bind to AGNN-containing stem-loops preferentially in vitro. In order to understand how Rnt1p recognizes its cognate processing sites, we have defined its minimal RNA-binding domain and determined its structure by solution NMR spectroscopy and X-ray crystallography. We observe a new carboxy-terminal helix following a canonical dsRBD structure. Removal of this helix reduces binding to Rnt1p substrates. The results suggest that this helix allows the Rnt1p dsRBD to bind to short RNA stem-loops by modulating the conformation of helix alpha1, a key RNA-recognition element of the dsRBD. | ||
==About this Structure== | ==About this Structure== | ||
1T4N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http:// | 1T4N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4N OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ares, M.]] | [[Category: Ares, M.]] | ||
[[Category: Edwards, T | [[Category: Edwards, T E.]] | ||
[[Category: Godin, K | [[Category: Godin, K S.]] | ||
[[Category: Graille, M.]] | [[Category: Graille, M.]] | ||
[[Category: Leeper, T | [[Category: Leeper, T C.]] | ||
[[Category: Leulliot, N.]] | [[Category: Leulliot, N.]] | ||
[[Category: Nagel, R | [[Category: Nagel, R J.]] | ||
[[Category: Quevillon-Cheruel, S.]] | [[Category: Quevillon-Cheruel, S.]] | ||
[[Category: Rozenkrants, N.]] | [[Category: Rozenkrants, N.]] | ||
[[Category: Sigurdsson, S | [[Category: Sigurdsson, S T.]] | ||
[[Category: Tilbeurgh, H | [[Category: Tilbeurgh, H van.]] | ||
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
[[Category: dsrbd]] | [[Category: dsrbd]] | ||
[[Category: rna-binding]] | [[Category: rna-binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:50 2008'' | ||
