1t5s: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1t5s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t5s, resolution 2.60Å" /> '''Structure of the (SR... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1t5s.gif|left|200px]]<br /><applet load="1t5s" size=" | [[Image:1t5s.gif|left|200px]]<br /><applet load="1t5s" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1t5s, resolution 2.60Å" /> | caption="1t5s, resolution 2.60Å" /> | ||
'''Structure of the (SR)Ca2+-ATPase Ca2-E1-AMPPCP form'''<br /> | '''Structure of the (SR)Ca2+-ATPase Ca2-E1-AMPPCP form'''<br /> | ||
==Overview== | ==Overview== | ||
A tight coupling between adenosine triphosphate (ATP) hydrolysis and | A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane. | ||
==About this Structure== | ==About this Structure== | ||
1T5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with CA, MG, K and ACP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http:// | 1T5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=ACP:'>ACP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5S OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Moller, J | [[Category: Moller, J V.]] | ||
[[Category: Nissen, P.]] | [[Category: Nissen, P.]] | ||
[[Category: Sorensen, T | [[Category: Sorensen, T L.M.]] | ||
[[Category: ACP]] | [[Category: ACP]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 29: | Line 29: | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:15 2008'' | ||
