1t7p: Difference between revisions
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New page: left|200px<br /><applet load="1t7p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t7p, resolution 2.2Å" /> '''T7 DNA POLYMERASE COM... |
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[[Image:1t7p.gif|left|200px]]<br /><applet load="1t7p" size=" | [[Image:1t7p.gif|left|200px]]<br /><applet load="1t7p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1t7p, resolution 2.2Å" /> | caption="1t7p, resolution 2.2Å" /> | ||
'''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''<br /> | '''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''<br /> | ||
==Overview== | ==Overview== | ||
DNA polymerases change their specificity for nucleotide substrates with | DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases. | ||
==About this Structure== | ==About this Structure== | ||
1T7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and DG3 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | 1T7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=DG3:'>DG3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Doublie, S.]] | [[Category: Doublie, S.]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
[[Category: Long, A | [[Category: Long, A M.]] | ||
[[Category: Richardson, C | [[Category: Richardson, C C.]] | ||
[[Category: Tabor, S.]] | [[Category: Tabor, S.]] | ||
[[Category: DG3]] | [[Category: DG3]] | ||
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[[Category: thioredoxin]] | [[Category: thioredoxin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:45 2008'' | ||
