1t7s: Difference between revisions

Line 1:

[[Image:1t7s.gif|left|200px]]<br /><applet load="1t7s" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1t7s.gif|left|200px]]<br /><applet load="1t7s" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1t7s, resolution 2.80Å" />

'''Structural Genomics of Caenorhabditis elegans: Structure of BAG-1 protein'''<br />

==Overview==

Binding of the BAG domain to the eukaryotic chaperone heat-shock protein, (Hsp70) promotes ATP-dependent release of the protein substrate from, Hsp70. Although the murine and human BAG domains have been shown to form, an antiparallel three-helix bundle, the Caenorhabditis elegans BAG domain, is formed by two antiparallel helices, while the third helix is extended, away and stabilized by crystal-packing interactions. A small beta-sheet, between helices 2 and 3 interferes with formation of the intramolecular, three-helix bundle. However, intermolecular three-helix bundles are, observed throughout the crystal packing and suggest that stable functional, dimers and tetramers can be formed in solution. The structure may, represent a new folding type of the BAG domain.

Binding of the BAG domain to the eukaryotic chaperone heat-shock protein (Hsp70) promotes ATP-dependent release of the protein substrate from Hsp70. Although the murine and human BAG domains have been shown to form an antiparallel three-helix bundle, the Caenorhabditis elegans BAG domain is formed by two antiparallel helices, while the third helix is extended away and stabilized by crystal-packing interactions. A small beta-sheet between helices 2 and 3 interferes with formation of the intramolecular three-helix bundle. However, intermolecular three-helix bundles are observed throughout the crystal packing and suggest that stable functional dimers and tetramers can be formed in solution. The structure may represent a new folding type of the BAG domain.

==About this Structure==

1T7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1T7S OCA].

1T7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7S OCA].

==Reference==

Line 15:

[[Category: Bunzel, R.]]

[[Category: Li, S.]]

[[Category: Luan, C.H.]]

[[Category: Luan, C H.]]

[[Category: Luo, M.]]

[[Category: Pruett, P.]]

[[Category: SECSG, Southeast.Collaboratory.for.Structural.Genomics.]]

[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]

[[Category: Schormann, N.]]

[[Category: Symersky, J.]]

Line 29:

[[Category: structural genomics]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 03:03:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:48 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1t7s.gif|left|200px]]<br /><applet load="1t7s" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1t7s.gif|left|200px]]<br /><applet load="1t7s" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1t7s, resolution 2.80&Aring;" />
 '''Structural Genomics of Caenorhabditis elegans: Structure of BAG-1 protein'''<br />
 ==Overview==
-Binding of the BAG domain to the eukaryotic chaperone heat-shock protein, (Hsp70) promotes ATP-dependent release of the protein substrate from, Hsp70. Although the murine and human BAG domains have been shown to form, an antiparallel three-helix bundle, the Caenorhabditis elegans BAG domain, is formed by two antiparallel helices, while the third helix is extended, away and stabilized by crystal-packing interactions. A small beta-sheet, between helices 2 and 3 interferes with formation of the intramolecular, three-helix bundle. However, intermolecular three-helix bundles are, observed throughout the crystal packing and suggest that stable functional, dimers and tetramers can be formed in solution. The structure may, represent a new folding type of the BAG domain.
+Binding of the BAG domain to the eukaryotic chaperone heat-shock protein (Hsp70) promotes ATP-dependent release of the protein substrate from Hsp70. Although the murine and human BAG domains have been shown to form an antiparallel three-helix bundle, the Caenorhabditis elegans BAG domain is formed by two antiparallel helices, while the third helix is extended away and stabilized by crystal-packing interactions. A small beta-sheet between helices 2 and 3 interferes with formation of the intramolecular three-helix bundle. However, intermolecular three-helix bundles are observed throughout the crystal packing and suggest that stable functional dimers and tetramers can be formed in solution. The structure may represent a new folding type of the BAG domain.
 ==About this Structure==
-T7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T7S OCA].
+T7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7S OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Bunzel, R.]]
 [[Category: Li, S.]]
-[[Category: Luan, C.H.]]
+[[Category: Luan, C H.]]
 [[Category: Luo, M.]]
 [[Category: Pruett, P.]]
-[[Category: SECSG, Southeast.Collaboratory.for.Structural.Genomics.]]
+[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
 [[Category: Schormann, N.]]
 [[Category: Symersky, J.]]
@@ Line 29: / Line 29: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:03:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:48 2008''