1thd: Difference between revisions

Line 1:

[[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1thd" />

'''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION'''<br />

==Overview==

Dengue virus, a member of the Flaviviridae family, has a surface composed, of 180 copies each of the envelope (E) glycoprotein and the membrane (M), protein. The crystal structure of an N-terminal fragment of E has been, determined and compared with a previously described structure. The primary, difference between these structures is a 10 degrees rotation about a hinge, relating the fusion domain DII to domains DI and DIII. These two rigid, body components were used for independent fitting of E into the, cryo-electron microscopy maps of both immature and mature dengue viruses., The fitted E structures in these two particles showed a difference of 27, degrees between the two components. Comparison of the E structure in its, postfusion state with that in the immature and mature virions shows a, rotation approximately around the same hinge. Flexibility of E is, apparently a functional requirement for assembly and infection of, flaviviruses.

Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses.

==About this Structure==

1THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1THD OCA].

1THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THD OCA].

==Reference==

Line 13:

[[Category: Dengue virus type 3]]

[[Category: Single protein]]

[[Category: Baker, T.S.]]

[[Category: Baker, T S.]]

[[Category: Clements, D.]]

[[Category: Kuhn, R.J.]]

[[Category: Kuhn, R J.]]

[[Category: Ogata, S.]]

[[Category: Rossmann, M.G.]]

[[Category: Rossmann, M G.]]

[[Category: Strauss, J.H.]]

[[Category: Strauss, J H.]]

[[Category: Zhang, W.]]

[[Category: Zhang, Y.]]

Line 29:

[[Category: icosahedral virus]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 03:15:~~50 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:31 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1thd" />
 '''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION'''<br />
 ==Overview==
-Dengue virus, a member of the Flaviviridae family, has a surface composed, of 180 copies each of the envelope (E) glycoprotein and the membrane (M), protein. The crystal structure of an N-terminal fragment of E has been, determined and compared with a previously described structure. The primary, difference between these structures is a 10 degrees rotation about a hinge, relating the fusion domain DII to domains DI and DIII. These two rigid, body components were used for independent fitting of E into the, cryo-electron microscopy maps of both immature and mature dengue viruses., The fitted E structures in these two particles showed a difference of 27, degrees between the two components. Comparison of the E structure in its, postfusion state with that in the immature and mature virions shows a, rotation approximately around the same hinge. Flexibility of E is, apparently a functional requirement for assembly and infection of, flaviviruses.
+Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses.
 ==About this Structure==
-THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1THD OCA].
+THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THD OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Dengue virus type 3]]
 [[Category: Single protein]]
-[[Category: Baker, T.S.]]
+[[Category: Baker, T S.]]
 [[Category: Clements, D.]]
-[[Category: Kuhn, R.J.]]
+[[Category: Kuhn, R J.]]
 [[Category: Ogata, S.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
-[[Category: Strauss, J.H.]]
+[[Category: Strauss, J H.]]
 [[Category: Zhang, W.]]
 [[Category: Zhang, Y.]]
@@ Line 29: / Line 29: @@
 [[Category: icosahedral virus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:15:50 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:31 2008''