1trj: Difference between revisions

Line 1:

[[Image:1trj.gif|left|200px]]<br /><applet load="1trj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1trj.gif|left|200px]]<br /><applet load="1trj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1trj, resolution 11.70Å" />

'''Homology Model of Yeast RACK1 Protein fitted into 11.7A cryo-EM map of Yeast 80S Ribosome'''<br />

==Overview==

RACK1 serves as a scaffold protein for a wide range of kinases and, membrane-bound receptors. It is a WD-repeat family protein and is, predicted to have a beta-propeller architecture with seven blades like a, Gbeta protein. Mass spectrometry studies have identified its association, with the small subunit of eukaryotic ribosomes and, most recently, it has, been shown to regulate initiation by recruiting protein kinase C to the, 40S subunit. Here we present the results of a cryo-EM study of the 80S, ribosome that positively locate RACK1 on the head region of the 40S, subunit, in the immediate vicinity of the mRNA exit channel. One face of, RACK1 exposes the WD-repeats as a platform for interactions with kinases, and receptors. Using this platform, RACK1 can recruit other proteins to, the ribosome.

RACK1 serves as a scaffold protein for a wide range of kinases and membrane-bound receptors. It is a WD-repeat family protein and is predicted to have a beta-propeller architecture with seven blades like a Gbeta protein. Mass spectrometry studies have identified its association with the small subunit of eukaryotic ribosomes and, most recently, it has been shown to regulate initiation by recruiting protein kinase C to the 40S subunit. Here we present the results of a cryo-EM study of the 80S ribosome that positively locate RACK1 on the head region of the 40S subunit, in the immediate vicinity of the mRNA exit channel. One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome.

==About this Structure==

1TRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA].

1TRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA].

==Reference==

Line 18:

[[Category: Nissen, P.]]

[[Category: Sengupta, J.]]

[[Category: Spahn, C.M.]]

[[Category: Spahn, C M.]]

[[Category: cryo-electron microscopy]]

[[Category: homology model]]

Line 24:

[[Category: ribosome]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:16:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1trj.gif|left|200px]]<br /><applet load="1trj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1trj.gif|left|200px]]<br /><applet load="1trj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1trj, resolution 11.70&Aring;" />
 '''Homology Model of Yeast RACK1 Protein fitted into 11.7A cryo-EM map of Yeast 80S Ribosome'''<br />
 ==Overview==
-RACK1 serves as a scaffold protein for a wide range of kinases and, membrane-bound receptors. It is a WD-repeat family protein and is, predicted to have a beta-propeller architecture with seven blades like a, Gbeta protein. Mass spectrometry studies have identified its association, with the small subunit of eukaryotic ribosomes and, most recently, it has, been shown to regulate initiation by recruiting protein kinase C to the, 40S subunit. Here we present the results of a cryo-EM study of the 80S, ribosome that positively locate RACK1 on the head region of the 40S, subunit, in the immediate vicinity of the mRNA exit channel. One face of, RACK1 exposes the WD-repeats as a platform for interactions with kinases, and receptors. Using this platform, RACK1 can recruit other proteins to, the ribosome.
+RACK1 serves as a scaffold protein for a wide range of kinases and membrane-bound receptors. It is a WD-repeat family protein and is predicted to have a beta-propeller architecture with seven blades like a Gbeta protein. Mass spectrometry studies have identified its association with the small subunit of eukaryotic ribosomes and, most recently, it has been shown to regulate initiation by recruiting protein kinase C to the 40S subunit. Here we present the results of a cryo-EM study of the 80S ribosome that positively locate RACK1 on the head region of the 40S subunit, in the immediate vicinity of the mRNA exit channel. One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome.
 ==About this Structure==
-TRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA].
+TRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA].
 ==Reference==
@@ Line 18: / Line 18: @@
 [[Category: Nissen, P.]]
 [[Category: Sengupta, J.]]
-[[Category: Spahn, C.M.]]
+[[Category: Spahn, C M.]]
 [[Category: cryo-electron microscopy]]
 [[Category: homology model]]
@@ Line 24: / Line 24: @@
 [[Category: ribosome]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:32:18 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:16:40 2008''