1u0l: Difference between revisions
New page: left|200px<br /><applet load="1u0l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u0l, resolution 2.80Å" /> '''Crystal structure of... |
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[[Image:1u0l.jpg|left|200px]]<br /><applet load="1u0l" size=" | [[Image:1u0l.jpg|left|200px]]<br /><applet load="1u0l" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1u0l, resolution 2.80Å" /> | caption="1u0l, resolution 2.80Å" /> | ||
'''Crystal structure of YjeQ from Thermotoga maritima'''<br /> | '''Crystal structure of YjeQ from Thermotoga maritima'''<br /> | ||
==Overview== | ==Overview== | ||
We have determined the crystal structure of the GDP complex of the YjeQ | We have determined the crystal structure of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known to bind to the ribosome, is composed of three domains: an N-terminal oligonucleotide/oligosaccharide-binding fold domain, a central GTPase domain, and a C-terminal zinc-finger domain. The crystal structure of TmYjeQ reveals two interesting domains: a circularly permutated GTPase domain and an unusual zinc-finger domain. The binding mode of GDP in the GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras proteins, a prototype GTPase. The N-terminal oligonucleotide/oligosaccharide-binding fold domain, together with the GTPase domain, forms the extended RNA-binding site. The C-terminal domain has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a DNA-repair protein, rad51 fragment. The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome. | ||
==About this Structure== | ==About this Structure== | ||
1U0L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ZN and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1U0L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0L OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
[[Category: BSGC, Berkeley | [[Category: BSGC, Berkeley Structural Genomics Center.]] | ||
[[Category: Jaru, J.]] | [[Category: Jaru, J.]] | ||
[[Category: Kim, R.]] | [[Category: Kim, R.]] | ||
[[Category: Kim, S | [[Category: Kim, S H.]] | ||
[[Category: Lou, Y.]] | [[Category: Lou, Y.]] | ||
[[Category: Shin, D | [[Category: Shin, D H.]] | ||
[[Category: Yokota, H.]] | [[Category: Yokota, H.]] | ||
[[Category: GDP]] | [[Category: GDP]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:24 2008'' | ||
Revision as of 13:19, 21 February 2008
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Crystal structure of YjeQ from Thermotoga maritima
Overview
We have determined the crystal structure of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known to bind to the ribosome, is composed of three domains: an N-terminal oligonucleotide/oligosaccharide-binding fold domain, a central GTPase domain, and a C-terminal zinc-finger domain. The crystal structure of TmYjeQ reveals two interesting domains: a circularly permutated GTPase domain and an unusual zinc-finger domain. The binding mode of GDP in the GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras proteins, a prototype GTPase. The N-terminal oligonucleotide/oligosaccharide-binding fold domain, together with the GTPase domain, forms the extended RNA-binding site. The C-terminal domain has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a DNA-repair protein, rad51 fragment. The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome.
About this Structure
1U0L is a Single protein structure of sequence from Thermotoga maritima with ZN and GDP as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain., Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13198-203. Epub 2004 Aug 26. PMID:15331784
Page seeded by OCA on Thu Feb 21 15:19:24 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Single protein
- Thermotoga maritima
- BSGC, Berkeley Structural Genomics Center.
- Jaru, J.
- Kim, R.
- Kim, S H.
- Lou, Y.
- Shin, D H.
- Yokota, H.
- GDP
- ZN
- Berkeley structural genomics center
- Bsgc structure funded by nih
- Gtpase
- Ob-fold
- Permutation
- Protein structure initiative
- Psi
- Structural genomics
- Zinc-finger
