1u34: Difference between revisions
New page: left|200px<br /><applet load="1u34" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u34" /> '''3D NMR structure of the first extracellular ... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1u34.gif|left|200px]]<br /><applet load="1u34" size=" | [[Image:1u34.gif|left|200px]]<br /><applet load="1u34" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1u34" /> | caption="1u34" /> | ||
'''3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor'''<br /> | '''3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor'''<br /> | ||
==Overview== | ==Overview== | ||
The corticotropin-releasing factor (CRF) ligand family has diverse effects | The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects are mediated by binding to CRF G protein-coupled receptors. We have determined the 3D NMR structure of the N-terminal extracellular domain (ECD1) of the mouse CRF receptor 2beta, which is the major ligand recognition domain, and identified its ligand binding site by chemical-shift perturbation experiments. The fold is identified as a short consensus repeat (SCR), a common protein interaction module. Mutagenesis reveals the integrity of the hormone-binding site in the full-length receptor. This study proposes that the ECD1 captures the C-terminal segment of the ligand, whose N terminus then penetrates into the transmembrane region of the receptor to initiate signaling. Key residues of SCR in the ECD1 are conserved in the G protein-coupled receptor subfamily, suggesting the SCR fold in all of the ECD1s of this subfamily. | ||
==About this Structure== | ==About this Structure== | ||
1U34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | 1U34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U34 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: DiGruccio, M | [[Category: DiGruccio, M R.]] | ||
[[Category: Grace, C | [[Category: Grace, C R.]] | ||
[[Category: Miller, C | [[Category: Miller, C L.]] | ||
[[Category: Perrin, M | [[Category: Perrin, M H.]] | ||
[[Category: Riek, R.]] | [[Category: Riek, R.]] | ||
[[Category: Rivier, J | [[Category: Rivier, J E.]] | ||
[[Category: Vale, W | [[Category: Vale, W W.]] | ||
[[Category: beta sheets and loops]] | [[Category: beta sheets and loops]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:08 2008'' | ||
Revision as of 13:20, 21 February 2008
|
3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor
Overview
The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects are mediated by binding to CRF G protein-coupled receptors. We have determined the 3D NMR structure of the N-terminal extracellular domain (ECD1) of the mouse CRF receptor 2beta, which is the major ligand recognition domain, and identified its ligand binding site by chemical-shift perturbation experiments. The fold is identified as a short consensus repeat (SCR), a common protein interaction module. Mutagenesis reveals the integrity of the hormone-binding site in the full-length receptor. This study proposes that the ECD1 captures the C-terminal segment of the ligand, whose N terminus then penetrates into the transmembrane region of the receptor to initiate signaling. Key residues of SCR in the ECD1 are conserved in the G protein-coupled receptor subfamily, suggesting the SCR fold in all of the ECD1s of this subfamily.
About this Structure
1U34 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
NMR structure and peptide hormone binding site of the first extracellular domain of a type B1 G protein-coupled receptor., Grace CR, Perrin MH, DiGruccio MR, Miller CL, Rivier JE, Vale WW, Riek R, Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12836-41. Epub 2004 Aug 23. PMID:15326300
Page seeded by OCA on Thu Feb 21 15:20:08 2008
