1u77: Difference between revisions

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[[Image:1u77.jpg|left|200px]]<br /><applet load="1u77" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1u77.jpg|left|200px]]<br /><applet load="1u77" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1u77, resolution 2.0Å" />

'''Crystal Structure of Ammonia Channel AmtB from E. Coli'''<br />

==Overview==

The first structure of an ammonia channel from the Amt/MEP/Rh protein, superfamily, determined to 1.35 angstrom resolution, shows it to be a, channel that spans the membrane 11 times. Two structurally similar halves, span the membrane with opposite polarity. Structures with and without, ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a, binding site for NH4+, and a 20 angstrom-long hydrophobic channel that, lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions, for NH3 are seen within the channel and use conserved histidines., Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged, NH3.

The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.

==About this Structure==

1U77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1U77 OCA].

1U77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U77 OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: III, J.O.Connell.]]

[[Category: III, J O.Connell.]]

[[Category: Khademi, S.]]

[[Category: Miercke, L.J.W.]]

[[Category: Miercke, L J.W.]]

[[Category: Remis, J.]]

[[Category: Robles-Colmenares, Y.]]

[[Category: Stroud, R.M.]]

[[Category: Stroud, R M.]]

[[Category: ammonia channel]]

[[Category: membrane channel]]

Line 24:

[[Category: transmembrane helices]]

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@@ Line 1: / Line 1: @@
-[[Image:1u77.jpg|left|200px]]<br /><applet load="1u77" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1u77.jpg|left|200px]]<br /><applet load="1u77" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1u77, resolution 2.0&Aring;" />
 '''Crystal Structure of Ammonia Channel AmtB from E. Coli'''<br />
 ==Overview==
-The first structure of an ammonia channel from the Amt/MEP/Rh protein, superfamily, determined to 1.35 angstrom resolution, shows it to be a, channel that spans the membrane 11 times. Two structurally similar halves, span the membrane with opposite polarity. Structures with and without, ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a, binding site for NH4+, and a 20 angstrom-long hydrophobic channel that, lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions, for NH3 are seen within the channel and use conserved histidines., Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged, NH3.
+The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.
 ==About this Structure==
-U77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U77 OCA].
+U77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U77 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: III, J.O.Connell.]]
+[[Category: III, J O.Connell.]]
 [[Category: Khademi, S.]]
-[[Category: Miercke, L.J.W.]]
+[[Category: Miercke, L J.W.]]
 [[Category: Remis, J.]]
 [[Category: Robles-Colmenares, Y.]]
-[[Category: Stroud, R.M.]]
+[[Category: Stroud, R M.]]
 [[Category: ammonia channel]]
 [[Category: membrane channel]]
@@ Line 24: / Line 24: @@
 [[Category: transmembrane helices]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:53:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:18 2008''