1uj1: Difference between revisions

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[[Image:1uj1.jpg|left|200px]]<br /><applet load="1uj1" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1uj1.jpg|left|200px]]<br /><applet load="1uj1" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1uj1, resolution 1.9Å" />

'''Crystal structure of SARS Coronavirus Main Proteinase (3CLpro)'''<br />

==Overview==

A newly identified severe acute respiratory syndrome coronavirus, (SARS-CoV), is the etiological agent responsible for the outbreak of SARS., The SARS-CoV main protease, which is a 33.8-kDa protease (also called the, 3C-like protease), plays a pivotal role in mediating viral replication and, transcription functions through extensive proteolytic processing of two, replicase polyproteins, pp1a (486 kDa) and pp1ab (790 kDa). Here, we, report the crystal structures of the SARS-CoV main protease at different, pH values and in complex with a specific inhibitor. The protease structure, has a fold that can be described as an augmented serine-protease, but with, a Cys-His at the active site. This series of crystal structures, which is, the first, to our knowledge, of any protein from the SARS virus, reveal, substantial pH-dependent conformational changes, and an unexpected mode of, inhibitor binding, providing a structural basis for rational drug design.

A newly identified severe acute respiratory syndrome coronavirus (SARS-CoV), is the etiological agent responsible for the outbreak of SARS. The SARS-CoV main protease, which is a 33.8-kDa protease (also called the 3C-like protease), plays a pivotal role in mediating viral replication and transcription functions through extensive proteolytic processing of two replicase polyproteins, pp1a (486 kDa) and pp1ab (790 kDa). Here, we report the crystal structures of the SARS-CoV main protease at different pH values and in complex with a specific inhibitor. The protease structure has a fold that can be described as an augmented serine-protease, but with a Cys-His at the active site. This series of crystal structures, which is the first, to our knowledge, of any protein from the SARS virus, reveal substantial pH-dependent conformational changes, and an unexpected mode of inhibitor binding, providing a structural basis for rational drug design.

==About this Structure==

1UJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1UJ1 OCA].

1UJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJ1 OCA].

==Reference==

Line 16:

[[Category: Bartlam, M.]]

[[Category: Ding, Y.]]

[[Category: Gao, G.F.]]

[[Category: Gao, G F.]]

[[Category: Hilgenfeld, R.]]

[[Category: Liu, Y.]]

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[[Category: anti-parallel b-barrel]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:09 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1uj1.jpg|left|200px]]<br /><applet load="1uj1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1uj1.jpg|left|200px]]<br /><applet load="1uj1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1uj1, resolution 1.9&Aring;" />
 '''Crystal structure of SARS Coronavirus Main Proteinase (3CLpro)'''<br />
 ==Overview==
-A newly identified severe acute respiratory syndrome coronavirus, (SARS-CoV), is the etiological agent responsible for the outbreak of SARS., The SARS-CoV main protease, which is a 33.8-kDa protease (also called the, 3C-like protease), plays a pivotal role in mediating viral replication and, transcription functions through extensive proteolytic processing of two, replicase polyproteins, pp1a (486 kDa) and pp1ab (790 kDa). Here, we, report the crystal structures of the SARS-CoV main protease at different, pH values and in complex with a specific inhibitor. The protease structure, has a fold that can be described as an augmented serine-protease, but with, a Cys-His at the active site. This series of crystal structures, which is, the first, to our knowledge, of any protein from the SARS virus, reveal, substantial pH-dependent conformational changes, and an unexpected mode of, inhibitor binding, providing a structural basis for rational drug design.
+A newly identified severe acute respiratory syndrome coronavirus (SARS-CoV), is the etiological agent responsible for the outbreak of SARS. The SARS-CoV main protease, which is a 33.8-kDa protease (also called the 3C-like protease), plays a pivotal role in mediating viral replication and transcription functions through extensive proteolytic processing of two replicase polyproteins, pp1a (486 kDa) and pp1ab (790 kDa). Here, we report the crystal structures of the SARS-CoV main protease at different pH values and in complex with a specific inhibitor. The protease structure has a fold that can be described as an augmented serine-protease, but with a Cys-His at the active site. This series of crystal structures, which is the first, to our knowledge, of any protein from the SARS virus, reveal substantial pH-dependent conformational changes, and an unexpected mode of inhibitor binding, providing a structural basis for rational drug design.
 ==About this Structure==
-UJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UJ1 OCA].
+UJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJ1 OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Bartlam, M.]]
 [[Category: Ding, Y.]]
-[[Category: Gao, G.F.]]
+[[Category: Gao, G F.]]
 [[Category: Hilgenfeld, R.]]
 [[Category: Liu, Y.]]
@@ Line 30: / Line 30: @@
 [[Category: anti-parallel b-barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:10:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:09 2008''