1upg: Difference between revisions

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[[Image:1upg.gif|left|200px]]<br /><applet load="1upg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1upg.gif|left|200px]]<br /><applet load="1upg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1upg, resolution 1.8Å" />

'''CRYSTAL STRUCTURE OF THE QUORUM-SENSING PROTEIN TRAM FROM AGROBACTERIUM TUMEFACIENS'''<br />

==Overview==

Transfer of the tumor-inducing plasmid in Agrobacterium tumefaciens is, controlled by a quorum-sensing system whose main components are the, transcriptional regulator TraR and its autoinducer. This system allows, bacteria to synchronize infection of the host plant when a "quorum" of, cells has been reached. TraM is an A. tumefaciens protein involved in the, regulation of this system because it binds to TraR and prevents it from, binding DNA. As a first step to understanding the molecular basis for the, regulation of TraR by TraM, we have determined the crystal structure of, TraM at 1.65 A resolution. This protein is packed as a dimer, with each, monomer consisting mainly of two antiparallel alpha helices. Monomers are, tightly associated, with a large hydrophobic area buried upon, dimerization. Secondly, we characterized the TraR-TraM complex in vitro., TraM (11.4 kDa, monomer molecular mass) binds tightly TraR (27 kDa, monomer molecular mass) forming a stable oligomeric complex that likely, accounts for two TraR and two TraM dimers.

Transfer of the tumor-inducing plasmid in Agrobacterium tumefaciens is controlled by a quorum-sensing system whose main components are the transcriptional regulator TraR and its autoinducer. This system allows bacteria to synchronize infection of the host plant when a "quorum" of cells has been reached. TraM is an A. tumefaciens protein involved in the regulation of this system because it binds to TraR and prevents it from binding DNA. As a first step to understanding the molecular basis for the regulation of TraR by TraM, we have determined the crystal structure of TraM at 1.65 A resolution. This protein is packed as a dimer, with each monomer consisting mainly of two antiparallel alpha helices. Monomers are tightly associated, with a large hydrophobic area buried upon dimerization. Secondly, we characterized the TraR-TraM complex in vitro. TraM (11.4 kDa, monomer molecular mass) binds tightly TraR (27 kDa, monomer molecular mass) forming a stable oligomeric complex that likely accounts for two TraR and two TraM dimers.

==About this Structure==

1UPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1UPG OCA].

1UPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPG OCA].

==Reference==

Line 13:

[[Category: Agrobacterium tumefaciens]]

[[Category: Single protein]]

[[Category: Marco, S.Di.]]

[[Category: Marco, S Di.]]

[[Category: Vannini, A.]]

[[Category: conjugation]]

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[[Category: transcription regulation]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:01 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1upg.gif|left|200px]]<br /><applet load="1upg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1upg.gif|left|200px]]<br /><applet load="1upg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1upg, resolution 1.8&Aring;" />
 '''CRYSTAL STRUCTURE OF THE QUORUM-SENSING PROTEIN TRAM FROM AGROBACTERIUM TUMEFACIENS'''<br />
 ==Overview==
-Transfer of the tumor-inducing plasmid in Agrobacterium tumefaciens is, controlled by a quorum-sensing system whose main components are the, transcriptional regulator TraR and its autoinducer. This system allows, bacteria to synchronize infection of the host plant when a "quorum" of, cells has been reached. TraM is an A. tumefaciens protein involved in the, regulation of this system because it binds to TraR and prevents it from, binding DNA. As a first step to understanding the molecular basis for the, regulation of TraR by TraM, we have determined the crystal structure of, TraM at 1.65 A resolution. This protein is packed as a dimer, with each, monomer consisting mainly of two antiparallel alpha helices. Monomers are, tightly associated, with a large hydrophobic area buried upon, dimerization. Secondly, we characterized the TraR-TraM complex in vitro., TraM (11.4 kDa, monomer molecular mass) binds tightly TraR (27 kDa, monomer molecular mass) forming a stable oligomeric complex that likely, accounts for two TraR and two TraM dimers.
+Transfer of the tumor-inducing plasmid in Agrobacterium tumefaciens is controlled by a quorum-sensing system whose main components are the transcriptional regulator TraR and its autoinducer. This system allows bacteria to synchronize infection of the host plant when a "quorum" of cells has been reached. TraM is an A. tumefaciens protein involved in the regulation of this system because it binds to TraR and prevents it from binding DNA. As a first step to understanding the molecular basis for the regulation of TraR by TraM, we have determined the crystal structure of TraM at 1.65 A resolution. This protein is packed as a dimer, with each monomer consisting mainly of two antiparallel alpha helices. Monomers are tightly associated, with a large hydrophobic area buried upon dimerization. Secondly, we characterized the TraR-TraM complex in vitro. TraM (11.4 kDa, monomer molecular mass) binds tightly TraR (27 kDa, monomer molecular mass) forming a stable oligomeric complex that likely accounts for two TraR and two TraM dimers.
 ==About this Structure==
-UPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UPG OCA].
+UPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPG OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Agrobacterium tumefaciens]]
 [[Category: Single protein]]
-[[Category: Marco, S.Di.]]
+[[Category: Marco, S Di.]]
 [[Category: Vannini, A.]]
 [[Category: conjugation]]
@@ Line 21: / Line 21: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:17:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:01 2008''