1usg: Difference between revisions

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[[Image:1usg.jpg|left|200px]]<br /><applet load="1usg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1usg.jpg|left|200px]]<br /><applet load="1usg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1usg, resolution 1.53Å" />

'''L-LEUCINE-BINDING PROTEIN, APO FORM'''<br />

==Overview==

The periplasmic leucine-binding protein is the primary receptor for the, leucine transport system in Escherichia coli. We report here the structure, of an open ligand-free form solved by molecular replacement and refined at, 1.5-A resolution. In addition, two closed ligand-bound structures of the, same protein are presented, a phenylalanine-bound form at 1.8 A and a, leucine-bound structure at a nominal resolution of 2.4 A. These structures, show the basis of this protein's ligand specificity, as well as, illustrating the conformational changes that are associated with ligand, binding. Comparison with earlier structures provides further information, about solution conformations, as well as the different specificity of the, closely related leucine/isoleucine/valine-binding protein.

The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.

==About this Structure==

1USG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1USG OCA].

1USG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USG OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Luck, L.A.]]

[[Category: Luck, L A.]]

[[Category: Magnusson, U.]]

[[Category: Mowbray, S.L.]]

[[Category: Mowbray, S L.]]

[[Category: Salopek-Sondi, B.]]

[[Category: abc transport systems]]

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[[Category: x-ray crystallography]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 04:18:~~22 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:46 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1usg.jpg|left|200px]]<br /><applet load="1usg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1usg.jpg|left|200px]]<br /><applet load="1usg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1usg, resolution 1.53&Aring;" />
 '''L-LEUCINE-BINDING PROTEIN, APO FORM'''<br />
 ==Overview==
-The periplasmic leucine-binding protein is the primary receptor for the, leucine transport system in Escherichia coli. We report here the structure, of an open ligand-free form solved by molecular replacement and refined at, 1.5-A resolution. In addition, two closed ligand-bound structures of the, same protein are presented, a phenylalanine-bound form at 1.8 A and a, leucine-bound structure at a nominal resolution of 2.4 A. These structures, show the basis of this protein's ligand specificity, as well as, illustrating the conformational changes that are associated with ligand, binding. Comparison with earlier structures provides further information, about solution conformations, as well as the different specificity of the, closely related leucine/isoleucine/valine-binding protein.
+The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.
 ==About this Structure==
-USG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1USG OCA].
+USG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USG OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Luck, L.A.]]
+[[Category: Luck, L A.]]
 [[Category: Magnusson, U.]]
-[[Category: Mowbray, S.L.]]
+[[Category: Mowbray, S L.]]
 [[Category: Salopek-Sondi, B.]]
 [[Category: abc transport systems]]
@@ Line 23: / Line 23: @@
 [[Category: x-ray crystallography]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:18:22 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:46 2008''