Isoelectric point: Difference between revisions

From Proteopedia
Jump to navigationJump to search
Wayne Decatur (talk | contribs)
No edit summary
Wayne Decatur (talk | contribs)
No edit summary
Line 5: Line 5:
*Second, go to the [http://www.embl-heidelberg.de/cgi/pi-wrapper.pl EMBL WWW Gateway to Isoelectric Point Service], paste your sequence in the box, and press the button.
*Second, go to the [http://www.embl-heidelberg.de/cgi/pi-wrapper.pl EMBL WWW Gateway to Isoelectric Point Service], paste your sequence in the box, and press the button.


*Warning: the sequence you paste in must be in UPPER CASE one letter code. If you paste in a lower case sequence, you'll get pI = 6.014999, which is for the backbone only, because it doesn't recognize lower case amino acids!
*'''Warning''': the sequence you paste in must be in UPPER CASE one letter code. If you paste in a lower case sequence, you'll get pI = 6.014999, which is for the backbone only, because it doesn't recognize lower case amino acids!