1xae: Difference between revisions

Line 1:

[[Image:1xae.gif|left|200px]]<br /><applet load="1xae" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xae.gif|left|200px]]<br /><applet load="1xae" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xae, resolution 2.7Å" />

'''Crystal structure of wild type yellow fluorescent protein zFP538 from Zoanthus'''<br />

==Overview==

Crystal structures of the tetrameric yellow-fluorescent protein zFP538, from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are, presented. The atomic models have been refined at 2.7 and 2.5 A, resolution, with final crystallographic R factors of 0.206 (R(free) =, 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent, stereochemistry. The fold of the protomer is very similar to that of green, (GFP) and red (DsRed) fluorescent proteins; however, evidence from, crystallography and mass spectrometry suggests that zFP538 contains a, three-ring chromophore derived from that of GFP. The yellow-emitting, species (lambda(em)(max) = 538 nm) is proposed to result from a, transimination reaction in which a transiently appearing DsRed-like, acylimine is attacked by the terminal amino group of lysine 66 to form a, new six-membered ring, cleaving the polypeptide backbone at the 65-66, position. This extends the chromophore conjugation by an additional double, bond compared to GFP, lowering the absorption and emission frequencies., Substitution of lysine 66 with aspartate or glutamate partially converts, zFP538 into a red-fluorescent protein, providing additional support for an, acylimine intermediate. The diverse and unexpected roles of the side chain, at position 66 give new insight into the chemistry of chromophore, maturation in the extended family of GFP-like proteins.

Crystal structures of the tetrameric yellow-fluorescent protein zFP538 from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are presented. The atomic models have been refined at 2.7 and 2.5 A resolution, with final crystallographic R factors of 0.206 (R(free) = 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent stereochemistry. The fold of the protomer is very similar to that of green (GFP) and red (DsRed) fluorescent proteins; however, evidence from crystallography and mass spectrometry suggests that zFP538 contains a three-ring chromophore derived from that of GFP. The yellow-emitting species (lambda(em)(max) = 538 nm) is proposed to result from a transimination reaction in which a transiently appearing DsRed-like acylimine is attacked by the terminal amino group of lysine 66 to form a new six-membered ring, cleaving the polypeptide backbone at the 65-66 position. This extends the chromophore conjugation by an additional double bond compared to GFP, lowering the absorption and emission frequencies. Substitution of lysine 66 with aspartate or glutamate partially converts zFP538 into a red-fluorescent protein, providing additional support for an acylimine intermediate. The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins.

==About this Structure==

1XAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XAE OCA].

1XAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAE OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Zoanthus sp.]]

[[Category: Anderson, D.C.]]

[[Category: Anderson, D C.]]

[[Category: Branchaud, B.]]

[[Category: Kallio, K.]]

[[Category: Lukyanov, K.A.]]

[[Category: Lukyanov, K A.]]

[[Category: Remington, S.J.]]

[[Category: Remington, S J.]]

[[Category: Wachter, R.M.]]

[[Category: Wachter, R M.]]

[[Category: Yarbrough, D.K.]]

[[Category: Yarbrough, D K.]]

[[Category: BME]]

[[Category: beta-barrel]]

Line 25:

[[Category: fluorescent protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 05:56:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:41 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1xae.gif|left|200px]]<br /><applet load="1xae" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xae.gif|left|200px]]<br /><applet load="1xae" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xae, resolution 2.7&Aring;" />
 '''Crystal structure of wild type yellow fluorescent protein zFP538 from Zoanthus'''<br />
 ==Overview==
-Crystal structures of the tetrameric yellow-fluorescent protein zFP538, from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are, presented. The atomic models have been refined at 2.7 and 2.5 A, resolution, with final crystallographic R factors of 0.206 (R(free) =, 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent, stereochemistry. The fold of the protomer is very similar to that of green, (GFP) and red (DsRed) fluorescent proteins; however, evidence from, crystallography and mass spectrometry suggests that zFP538 contains a, three-ring chromophore derived from that of GFP. The yellow-emitting, species (lambda(em)(max) = 538 nm) is proposed to result from a, transimination reaction in which a transiently appearing DsRed-like, acylimine is attacked by the terminal amino group of lysine 66 to form a, new six-membered ring, cleaving the polypeptide backbone at the 65-66, position. This extends the chromophore conjugation by an additional double, bond compared to GFP, lowering the absorption and emission frequencies., Substitution of lysine 66 with aspartate or glutamate partially converts, zFP538 into a red-fluorescent protein, providing additional support for an, acylimine intermediate. The diverse and unexpected roles of the side chain, at position 66 give new insight into the chemistry of chromophore, maturation in the extended family of GFP-like proteins.
+Crystal structures of the tetrameric yellow-fluorescent protein zFP538 from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are presented. The atomic models have been refined at 2.7 and 2.5 A resolution, with final crystallographic R factors of 0.206 (R(free) = 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent stereochemistry. The fold of the protomer is very similar to that of green (GFP) and red (DsRed) fluorescent proteins; however, evidence from crystallography and mass spectrometry suggests that zFP538 contains a three-ring chromophore derived from that of GFP. The yellow-emitting species (lambda(em)(max) = 538 nm) is proposed to result from a transimination reaction in which a transiently appearing DsRed-like acylimine is attacked by the terminal amino group of lysine 66 to form a new six-membered ring, cleaving the polypeptide backbone at the 65-66 position. This extends the chromophore conjugation by an additional double bond compared to GFP, lowering the absorption and emission frequencies. Substitution of lysine 66 with aspartate or glutamate partially converts zFP538 into a red-fluorescent protein, providing additional support for an acylimine intermediate. The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins.
 ==About this Structure==
-XAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XAE OCA].
+XAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAE OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Zoanthus sp.]]
-[[Category: Anderson, D.C.]]
+[[Category: Anderson, D C.]]
 [[Category: Branchaud, B.]]
 [[Category: Kallio, K.]]
-[[Category: Lukyanov, K.A.]]
+[[Category: Lukyanov, K A.]]
-[[Category: Remington, S.J.]]
+[[Category: Remington, S J.]]
-[[Category: Wachter, R.M.]]
+[[Category: Wachter, R M.]]
-[[Category: Yarbrough, D.K.]]
+[[Category: Yarbrough, D K.]]
 [[Category: BME]]
 [[Category: beta-barrel]]
@@ Line 25: / Line 25: @@
 [[Category: fluorescent protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:56:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:41 2008''