1xfn: Difference between revisions

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[[Image:1xfn.gif|left|200px]]<br /><applet load="1xfn" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xfn.gif|left|200px]]<br /><applet load="1xfn" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xfn" />

'''NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part'''<br />

==Overview==

The N-terminally truncated variant of photoactive yellow protein, (Delta25-PYP) undergoes a very similar photocycle as the corresponding, wild-type protein (WT-PYP), although the lifetime of its light-illuminated, (pB) state is much longer. This has allowed determination of the structure, of both its dark- (pG) as well as its pB-state in solution by nuclear, magnetic resonance (NMR) spectroscopy. The pG structure shows a, well-defined fold, similar to WT-PYP and the X-ray structure of the pG, state of Delta25-PYP. In the long-lived photocycle intermediate pB, the, central beta sheet is still intact, as well as a small part of one alpha, helix. The remainder of pB is unfolded and highly flexible, as evidenced, by results from proton-deuterium exchange and NMR relaxation studies., Thus, the partially unfolded nature of the presumed signaling state of PYP, in solution, as suggested previously, has now been structurally, demonstrated.

The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.

==About this Structure==

1XFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA].

1XFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA].

==Reference==

Line 15:

[[Category: Bernard, C.]]

[[Category: Boelens, R.]]

[[Category: Derix, N.M.]]

[[Category: Derix, N M.]]

[[Category: Hellingwerf, K.J.]]

[[Category: Hellingwerf, K J.]]

[[Category: Horst, M.A.van.der.]]

[[Category: Horst, M A.van der.]]

[[Category: Houben, K.]]

[[Category: Kaptein, R.]]

[[Category: Marks, D.]]

[[Category: Nuland, N.A.van.]]

[[Category: Nuland, N A.van.]]

[[Category: HC4]]

[[Category: pas domain]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:02:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:54:16 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xfn.gif|left|200px]]<br /><applet load="1xfn" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xfn.gif|left|200px]]<br /><applet load="1xfn" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xfn" />
 '''NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part'''<br />
 ==Overview==
-The N-terminally truncated variant of photoactive yellow protein, (Delta25-PYP) undergoes a very similar photocycle as the corresponding, wild-type protein (WT-PYP), although the lifetime of its light-illuminated, (pB) state is much longer. This has allowed determination of the structure, of both its dark- (pG) as well as its pB-state in solution by nuclear, magnetic resonance (NMR) spectroscopy. The pG structure shows a, well-defined fold, similar to WT-PYP and the X-ray structure of the pG, state of Delta25-PYP. In the long-lived photocycle intermediate pB, the, central beta sheet is still intact, as well as a small part of one alpha, helix. The remainder of pB is unfolded and highly flexible, as evidenced, by results from proton-deuterium exchange and NMR relaxation studies., Thus, the partially unfolded nature of the presumed signaling state of PYP, in solution, as suggested previously, has now been structurally, demonstrated.
+The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.
 ==About this Structure==
-XFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA].
+XFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Bernard, C.]]
 [[Category: Boelens, R.]]
-[[Category: Derix, N.M.]]
+[[Category: Derix, N M.]]
-[[Category: Hellingwerf, K.J.]]
+[[Category: Hellingwerf, K J.]]
-[[Category: Horst, M.A.van.der.]]
+[[Category: Horst, M A.van der.]]
 [[Category: Houben, K.]]
 [[Category: Kaptein, R.]]
 [[Category: Marks, D.]]
-[[Category: Nuland, N.A.van.]]
+[[Category: Nuland, N A.van.]]
 [[Category: HC4]]
 [[Category: pas domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:02:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:54:16 2008''