1xjt: Difference between revisions
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New page: left|200px<br /><applet load="1xjt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xjt, resolution 1.750Å" /> '''Crystal structure o... |
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[[Image:1xjt.gif|left|200px]]<br /><applet load="1xjt" size=" | [[Image:1xjt.gif|left|200px]]<br /><applet load="1xjt" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1xjt, resolution 1.750Å" /> | caption="1xjt, resolution 1.750Å" /> | ||
'''Crystal structure of active form of P1 phage endolysin Lyz'''<br /> | '''Crystal structure of active form of P1 phage endolysin Lyz'''<br /> | ||
==Overview== | ==Overview== | ||
The P1 lysozyme Lyz is secreted to the periplasm of Escherichia coli and | The P1 lysozyme Lyz is secreted to the periplasm of Escherichia coli and accumulates in an inactive membrane-tethered form. Genetic and biochemical experiments show that, when released from the bilayer, Lyz is activated by an intramolecular thiol-disulfide isomerization, which requires a cysteine in its N-terminal SAR (signal-arrest-release) domain. Crystal structures confirm the alternative disulfide linkages in the two forms of Lyz and reveal dramatic conformational differences in the catalytic domain. Thus, the exported P1 endolysin is kept inactive by three levels of control-topological, conformational, and covalent-until its release from the membrane is triggered by the P1 holin. | ||
==About this Structure== | ==About this Structure== | ||
1XJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 1XJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJT OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arockiasamy, A.]] | [[Category: Arockiasamy, A.]] | ||
[[Category: Sacchettini, J | [[Category: Sacchettini, J C.]] | ||
[[Category: CIT]] | [[Category: CIT]] | ||
[[Category: open conformation]] | [[Category: open conformation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:55:30 2008'' | ||
