1xs9: Difference between revisions
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New page: left|200px<br /><applet load="1xs9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xs9" /> '''A MODEL OF THE TERNARY COMPLEX FORMED BETWEE... |
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[[Image:1xs9.gif|left|200px]]<br /><applet load="1xs9" size=" | [[Image:1xs9.gif|left|200px]]<br /><applet load="1xs9" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA'''<br /> | '''A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA'''<br /> | ||
==Overview== | ==Overview== | ||
The transcriptional activator, MarA, interacts with RNA polymerase (RNAP) | The transcriptional activator, MarA, interacts with RNA polymerase (RNAP) to activate promoters of the mar regulon. Here, we identify the interacting surfaces of MarA and of the carboxy-terminal domain of the alpha subunit of RNAP (alpha-CTD) by NMR-based chemical shift mapping. Spectral changes were monitored for a MarA-DNA complex upon titration with alpha-CTD, and for alpha-CTD upon titration with MarA-DNA. The mapping results were confirmed by mutational studies and retention chromatography. A model of the ternary complex shows that alpha-CTD uses a '265-like determinant' to contact MarA at a surface distant from the DNA. This is unlike the interaction of alpha-CTD with the CRP or Fis activators where the '265 determinant' contacts DNA while another surface of the same alpha-CTD molecule contacts the activator. These results reveal a new versatility for alpha-CTD in transcriptional activation. | ||
==About this Structure== | ==About this Structure== | ||
1XS9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure | 1XS9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1TI9. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dangi, B.]] | [[Category: Dangi, B.]] | ||
[[Category: Gronenborn, A | [[Category: Gronenborn, A M.]] | ||
[[Category: Martin, R | [[Category: Martin, R G.]] | ||
[[Category: Rosner, J | [[Category: Rosner, J L.]] | ||
[[Category: mara]] | [[Category: mara]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
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[[Category: ternary complex]] | [[Category: ternary complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:08 2008'' | ||
