1xsi: Difference between revisions

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[[Image:1xsi.gif|left|200px]]<br /><applet load="1xsi" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xsi.gif|left|200px]]<br /><applet load="1xsi" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xsi, resolution 2.20Å" />

'''Structure of a Family 31 alpha glycosidase'''<br />

==Overview==

We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a, 5-fluoroxylopyranosyl-enzyme intermediate via reaction with, 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure, shows an intimately associated hexamer with structural elements from, several monomers converging at each of the six active sites. Our kinetic, and mass spectrometry analyses verified several of the features observed, in our structural data, including a covalent linkage from the carboxylate, side chain of the identified nucleophile Asp(416) to C-1 of the sugar, ring. Structure-based sequence comparison of YicI with the mammalian, alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase, predicts a high level of structural similarity and provides a foundation, for understanding the various mutations of these enzymes that elicit human, disease.

We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.

==About this Structure==

1XSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, MPO and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA].

1XSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MPO:'>MPO</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Kim, Y.W.]]

[[Category: Kim, Y W.]]

[[Category: Lee, S.S.]]

[[Category: Lee, S S.]]

[[Category: Lovering, A.L.]]

[[Category: Lovering, A L.]]

[[Category: Strynadka, N.C.]]

[[Category: Strynadka, N C.]]

[[Category: Withers, S.G.]]

[[Category: Withers, S G.]]

[[Category: ACY]]

[[Category: MPO]]

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[[Category: beta(8)alpha(8) barrel and beta sandwich domains]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:18:~~59 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:13 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xsi.gif|left|200px]]<br /><applet load="1xsi" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xsi.gif|left|200px]]<br /><applet load="1xsi" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xsi, resolution 2.20&Aring;" />
 '''Structure of a Family 31 alpha glycosidase'''<br />
 ==Overview==
-We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a, 5-fluoroxylopyranosyl-enzyme intermediate via reaction with, 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure, shows an intimately associated hexamer with structural elements from, several monomers converging at each of the six active sites. Our kinetic, and mass spectrometry analyses verified several of the features observed, in our structural data, including a covalent linkage from the carboxylate, side chain of the identified nucleophile Asp(416) to C-1 of the sugar, ring. Structure-based sequence comparison of YicI with the mammalian, alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase, predicts a high level of structural similarity and provides a foundation, for understanding the various mutations of these enzymes that elicit human, disease.
+We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.
 ==About this Structure==
-XSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, MPO and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA].
+XSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MPO:'>MPO</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Kim, Y.W.]]
+[[Category: Kim, Y W.]]
-[[Category: Lee, S.S.]]
+[[Category: Lee, S S.]]
-[[Category: Lovering, A.L.]]
+[[Category: Lovering, A L.]]
-[[Category: Strynadka, N.C.]]
+[[Category: Strynadka, N C.]]
-[[Category: Withers, S.G.]]
+[[Category: Withers, S G.]]
 [[Category: ACY]]
 [[Category: MPO]]
@@ Line 23: / Line 23: @@
 [[Category: beta(8)alpha(8) barrel and beta sandwich domains]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:18:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:13 2008''