1xt6: Difference between revisions

Line 1:

[[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xt6, resolution 1.80Å" />

'''S35C Flavodoxin Mutant in the semiquinone state'''<br />

==Overview==

The crystallographic structure of an engineered flavodoxin mutant from, Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was, used to substitute serine 35 with a cysteine to provide a possible, covalent linkage. The crystal structure of the semiquinone form of this, mutant is similar to the corresponding oxidation state of the wild-type, flavodoxin. Analysis of the structural changes reveals the interaction, between N(5)H of the flavin and the carbonyl O atom of Gly61 to be, critical for modulation of the electrochemical properties of the protein.

The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.

==About this Structure==

1XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].

1XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].

==Reference==

Line 20:

[[Category: Marchini, N.]]

[[Category: Meneghetti, F.]]

[[Category: Rossi, G.L.]]

[[Category: Rossi, G L.]]

[[Category: Sassone, C.]]

[[Category: FMN]]

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[[Category: s35c]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xt6, resolution 1.80&Aring;" />
 '''S35C Flavodoxin Mutant in the semiquinone state'''<br />
 ==Overview==
-The crystallographic structure of an engineered flavodoxin mutant from, Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was, used to substitute serine 35 with a cysteine to provide a possible, covalent linkage. The crystal structure of the semiquinone form of this, mutant is similar to the corresponding oxidation state of the wild-type, flavodoxin. Analysis of the structural changes reveals the interaction, between N(5)H of the flavin and the carbonyl O atom of Gly61 to be, critical for modulation of the electrochemical properties of the protein.
+The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
 ==About this Structure==
-XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].
+XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: Marchini, N.]]
 [[Category: Meneghetti, F.]]
-[[Category: Rossi, G.L.]]
+[[Category: Rossi, G L.]]
 [[Category: Sassone, C.]]
 [[Category: FMN]]
@@ Line 28: / Line 28: @@
 [[Category: s35c]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:19:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:23 2008''