1y2y: Difference between revisions

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[[Image:1y2y.gif|left|200px]]<br /><applet load="1y2y" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1y2y.gif|left|200px]]<br /><applet load="1y2y" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1y2y" />

'''Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy'''<br />

==Overview==

The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the, modification of uridine to pseudouridine at conserved sites in rRNA. The, H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core, proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in, yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins, that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We, have determined the solution structure of an RNA hairpin derived from the, human U65 box H/ACA snoRNA including the pseudouridylation pocket and, adjacent stems, providing the first three-dimensional structural, information on these H/ACA snoRNAs. We have also determined the structure, of Nop10p and investigated its interaction with RNA using NMR, spectroscopy. Nop10p contains a structurally well-defined N-terminal, region composed of a beta-hairpin, and the rest of the protein lacks a, globular structure. Chemical shift mapping of the interaction of RNA, constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the, beta-hairpin binds weakly but specifically to RNA. The unstructured region, of Nop10p likely interacts with Cbf5p.

The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the modification of uridine to pseudouridine at conserved sites in rRNA. The H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We have determined the solution structure of an RNA hairpin derived from the human U65 box H/ACA snoRNA including the pseudouridylation pocket and adjacent stems, providing the first three-dimensional structural information on these H/ACA snoRNAs. We have also determined the structure of Nop10p and investigated its interaction with RNA using NMR spectroscopy. Nop10p contains a structurally well-defined N-terminal region composed of a beta-hairpin, and the rest of the protein lacks a globular structure. Chemical shift mapping of the interaction of RNA constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the beta-hairpin binds weakly but specifically to RNA. The unstructured region of Nop10p likely interacts with Cbf5p.

==About this Structure==

1Y2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA].

1Y2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA].

==Reference==

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[[Category: snornp]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:31:~~51 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:01:17 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1y2y.gif|left|200px]]<br /><applet load="1y2y" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1y2y.gif|left|200px]]<br /><applet load="1y2y" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1y2y" />
 '''Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy'''<br />
 ==Overview==
-The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the, modification of uridine to pseudouridine at conserved sites in rRNA. The, H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core, proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in, yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins, that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We, have determined the solution structure of an RNA hairpin derived from the, human U65 box H/ACA snoRNA including the pseudouridylation pocket and, adjacent stems, providing the first three-dimensional structural, information on these H/ACA snoRNAs. We have also determined the structure, of Nop10p and investigated its interaction with RNA using NMR, spectroscopy. Nop10p contains a structurally well-defined N-terminal, region composed of a beta-hairpin, and the rest of the protein lacks a, globular structure. Chemical shift mapping of the interaction of RNA, constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the, beta-hairpin binds weakly but specifically to RNA. The unstructured region, of Nop10p likely interacts with Cbf5p.
+The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the modification of uridine to pseudouridine at conserved sites in rRNA. The H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We have determined the solution structure of an RNA hairpin derived from the human U65 box H/ACA snoRNA including the pseudouridylation pocket and adjacent stems, providing the first three-dimensional structural information on these H/ACA snoRNAs. We have also determined the structure of Nop10p and investigated its interaction with RNA using NMR spectroscopy. Nop10p contains a structurally well-defined N-terminal region composed of a beta-hairpin, and the rest of the protein lacks a globular structure. Chemical shift mapping of the interaction of RNA constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the beta-hairpin binds weakly but specifically to RNA. The unstructured region of Nop10p likely interacts with Cbf5p.
 ==About this Structure==
-Y2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA].
+Y2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: snornp]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:31:51 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:01:17 2008''