1y5i: Difference between revisions
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New page: left|200px<br /><applet load="1y5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y5i, resolution 1.90Å" /> '''The crystal structur... |
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[[Image:1y5i.gif|left|200px]]<br /><applet load="1y5i" size=" | [[Image:1y5i.gif|left|200px]]<br /><applet load="1y5i" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1y5i, resolution 1.90Å" /> | caption="1y5i, resolution 1.90Å" /> | ||
'''The crystal structure of the NarGHI mutant NarI-K86A'''<br /> | '''The crystal structure of the NarGHI mutant NarI-K86A'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in | The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane. | ||
==About this Structure== | ==About this Structure== | ||
1Y5I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MD1, 6MO, HEM, SF4, F3S, AGA and 3PH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http:// | 1Y5I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MD1:'>MD1</scene>, <scene name='pdbligand=6MO:'>6MO</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=F3S:'>F3S</scene>, <scene name='pdbligand=AGA:'>AGA</scene> and <scene name='pdbligand=3PH:'>3PH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y5I OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Nitrate reductase]] | [[Category: Nitrate reductase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bertero, M | [[Category: Bertero, M G.]] | ||
[[Category: Blasco, F.]] | [[Category: Blasco, F.]] | ||
[[Category: Boroumand, N.]] | [[Category: Boroumand, N.]] | ||
[[Category: Ginet, N.]] | [[Category: Ginet, N.]] | ||
[[Category: Palak, M.]] | [[Category: Palak, M.]] | ||
[[Category: Rothery, R | [[Category: Rothery, R A.]] | ||
[[Category: Strynadka, N | [[Category: Strynadka, N C.J.]] | ||
[[Category: Weiner, J | [[Category: Weiner, J H.]] | ||
[[Category: 3PH]] | [[Category: 3PH]] | ||
[[Category: 6MO]] | [[Category: 6MO]] | ||
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[[Category: q-site]] | [[Category: q-site]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:02:00 2008'' | ||
