1yo4: Difference between revisions

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[[Image:1yo4.gif|left|200px]]<br /><applet load="1yo4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1yo4.gif|left|200px]]<br /><applet load="1yo4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1yo4" />

'''Solution Structure of the SARS Coronavirus ORF 7a coded X4 protein'''<br />

==Overview==

The SARS related Coronavirus genome contains a variety of novel accessory, genes. One of these, called ORF7a or ORF8, code for a protein, known as, 7a, U122 or X4. We set out to determine the three-dimensional structure of, the soluble ectodomain of this type-I transmembrane protein by nuclear, magnetic resonance spectroscopy. The fold of the protein is the first, member of a further variation of the immunoglobulin like beta-sandwich, fold. Because X4 does not reveal significant sequence homologies to, proteins in the data bases, we carried out a structure based similarity, search for proteins with known function. High structural similarity to Dl, domains of ICAM-1 and ICAM-2, and common features in amino acid sequence, between X4 and ICAM-1, suggest X4 to possess binding activity for the, alpha(L) integrin I domain of LFA-1. Further, based on this structure, based prediction, potential functions of X4 in virus replication and, pathogenesis are discussed.

The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectroscopy. The fold of the protein is the first member of a further variation of the immunoglobulin like beta-sandwich fold. Because X4 does not reveal significant sequence homologies to proteins in the data bases, we carried out a structure based similarity search for proteins with known function. High structural similarity to Dl domains of ICAM-1 and ICAM-2, and common features in amino acid sequence between X4 and ICAM-1, suggest X4 to possess binding activity for the alpha(L) integrin I domain of LFA-1. Further, based on this structure based prediction, potential functions of X4 in virus replication and pathogenesis are discussed.

==About this Structure==

1YO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1YO4 OCA].

1YO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YO4 OCA].

==Reference==

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[[Category: immunoglobulin-like domain]]

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@@ Line 1: / Line 1: @@
-[[Image:1yo4.gif|left|200px]]<br /><applet load="1yo4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yo4.gif|left|200px]]<br /><applet load="1yo4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1yo4" />
 '''Solution Structure of the SARS Coronavirus ORF 7a coded X4 protein'''<br />
 ==Overview==
-The SARS related Coronavirus genome contains a variety of novel accessory, genes. One of these, called ORF7a or ORF8, code for a protein, known as, 7a, U122 or X4. We set out to determine the three-dimensional structure of, the soluble ectodomain of this type-I transmembrane protein by nuclear, magnetic resonance spectroscopy. The fold of the protein is the first, member of a further variation of the immunoglobulin like beta-sandwich, fold. Because X4 does not reveal significant sequence homologies to, proteins in the data bases, we carried out a structure based similarity, search for proteins with known function. High structural similarity to Dl, domains of ICAM-1 and ICAM-2, and common features in amino acid sequence, between X4 and ICAM-1, suggest X4 to possess binding activity for the, alpha(L) integrin I domain of LFA-1. Further, based on this structure, based prediction, potential functions of X4 in virus replication and, pathogenesis are discussed.
+The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectroscopy. The fold of the protein is the first member of a further variation of the immunoglobulin like beta-sandwich fold. Because X4 does not reveal significant sequence homologies to proteins in the data bases, we carried out a structure based similarity search for proteins with known function. High structural similarity to Dl domains of ICAM-1 and ICAM-2, and common features in amino acid sequence between X4 and ICAM-1, suggest X4 to possess binding activity for the alpha(L) integrin I domain of LFA-1. Further, based on this structure based prediction, potential functions of X4 in virus replication and pathogenesis are discussed.
 ==About this Structure==
-YO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YO4 OCA].
+YO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YO4 OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: immunoglobulin-like domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:56:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:23 2008''