1yqw: Difference between revisions
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New page: left|200px<br /><applet load="1yqw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yqw, resolution 1.83Å" /> '''Structure of the Oxi... |
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[[Image:1yqw.gif|left|200px]]<br /><applet load="1yqw" size=" | [[Image:1yqw.gif|left|200px]]<br /><applet load="1yqw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1yqw, resolution 1.83Å" /> | caption="1yqw, resolution 1.83Å" /> | ||
'''Structure of the Oxidized Unready Form of Ni-Fe Hydrogenase'''<br /> | '''Structure of the Oxidized Unready Form of Ni-Fe Hydrogenase'''<br /> | ||
==Overview== | ==Overview== | ||
[NiFe] hydrogenases catalyze the reversible heterolytic cleavage of | [NiFe] hydrogenases catalyze the reversible heterolytic cleavage of molecular hydrogen. Several oxidized, inactive states of these enzymes are known that are distinguishable by their very different activation properties. So far, the structural basis for this difference has not been understood because of lack of relevant crystallographic data. Here, we present the crystal structure of the ready Ni-B state of Desulfovibrio fructosovorans [NiFe] hydrogenase and show it to have a putative mu-hydroxo Ni-Fe bridging ligand at the active site. On the other hand, a new, improved refinement procedure of the X-ray diffraction data obtained for putative unready Ni-A/Ni-SU states resulted in a more elongated electron density for the bridging ligand, suggesting that it is a diatomic species. The slow activation of the Ni-A state, compared with the rapid activation of the Ni-B state, is therefore proposed to result from the different chemical nature of the ligands in the two oxidized species. Our results along with very recent electrochemical studies suggest that the diatomic ligand could be hydro-peroxide. | ||
==About this Structure== | ==About this Structure== | ||
1YQW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_fructosovorans Desulfovibrio fructosovorans] with NI, FE2, BCT, MG, SF4, F3S, FCO, PER and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c3_hydrogenase Cytochrome-c3 hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.2.1 1.12.2.1] Full crystallographic information is available from [http:// | 1YQW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_fructosovorans Desulfovibrio fructosovorans] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=BCT:'>BCT</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=F3S:'>F3S</scene>, <scene name='pdbligand=FCO:'>FCO</scene>, <scene name='pdbligand=PER:'>PER</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c3_hydrogenase Cytochrome-c3 hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.2.1 1.12.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YQW OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ni-fe hydrogenase unready state]] | [[Category: ni-fe hydrogenase unready state]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:10 2008'' | ||
