1yrg: Difference between revisions

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[[Image:1yrg.gif|left|200px]]<br /><applet load="1yrg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1yrg.gif|left|200px]]<br /><applet load="1yrg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1yrg, resolution 2.66Å" />

'''THE CRYSTAL STRUCTURE OF RNA1P: A NEW FOLD FOR A GTPASE-ACTIVATING PROTEIN'''<br />

==Overview==

rna1p is the Schizosaccharomyces pombe ortholog of the mammalian, GTPase-activating protein (GAP) of Ran. Both proteins are essential for, nuclear transport. Here, we report the crystal structure of rna1p at 2.66, A resolution. It contains 11 leucine-rich repeats that adopt the, nonglobular shape of a crescent, bearing no resemblance to RhoGAP or, RasGAP. The invariant residues of RanGAP form a contiguous surface, strongly indicating the Ran-binding interface. Alanine mutations identify, Arg-74 as a critical residue for GTP hydrolysis. In contrast to RasGAP and, RhoGAP, Arg-74 could be substituted by lysine and contributed, significantly to the binding of Ran. Therefore, we suggest a GAP mechanism, for rna1p, which constitutes a variation of the arginine finger mechanism, found for Ras GAP and RhoGAP.

rna1p is the Schizosaccharomyces pombe ortholog of the mammalian GTPase-activating protein (GAP) of Ran. Both proteins are essential for nuclear transport. Here, we report the crystal structure of rna1p at 2.66 A resolution. It contains 11 leucine-rich repeats that adopt the nonglobular shape of a crescent, bearing no resemblance to RhoGAP or RasGAP. The invariant residues of RanGAP form a contiguous surface, strongly indicating the Ran-binding interface. Alanine mutations identify Arg-74 as a critical residue for GTP hydrolysis. In contrast to RasGAP and RhoGAP, Arg-74 could be substituted by lysine and contributed significantly to the binding of Ran. Therefore, we suggest a GAP mechanism for rna1p, which constitutes a variation of the arginine finger mechanism found for Ras GAP and RhoGAP.

==About this Structure==

1YRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA].

1YRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA].

==Reference==

Line 15:

[[Category: Becker, J.]]

[[Category: Drell, T.]]

[[Category: Hillig, R.C.]]

[[Category: Hillig, R C.]]

[[Category: Renault, L.]]

[[Category: Vetter, I.R.]]

[[Category: Vetter, I R.]]

[[Category: Wittinghofer, A.]]

[[Category: gap]]

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[[Category: twinning]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:00:~~07 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:24 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1yrg.gif|left|200px]]<br /><applet load="1yrg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yrg.gif|left|200px]]<br /><applet load="1yrg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1yrg, resolution 2.66&Aring;" />
 '''THE CRYSTAL STRUCTURE OF RNA1P: A NEW FOLD FOR A GTPASE-ACTIVATING PROTEIN'''<br />
 ==Overview==
-rna1p is the Schizosaccharomyces pombe ortholog of the mammalian, GTPase-activating protein (GAP) of Ran. Both proteins are essential for, nuclear transport. Here, we report the crystal structure of rna1p at 2.66, A resolution. It contains 11 leucine-rich repeats that adopt the, nonglobular shape of a crescent, bearing no resemblance to RhoGAP or, RasGAP. The invariant residues of RanGAP form a contiguous surface, strongly indicating the Ran-binding interface. Alanine mutations identify, Arg-74 as a critical residue for GTP hydrolysis. In contrast to RasGAP and, RhoGAP, Arg-74 could be substituted by lysine and contributed, significantly to the binding of Ran. Therefore, we suggest a GAP mechanism, for rna1p, which constitutes a variation of the arginine finger mechanism, found for Ras GAP and RhoGAP.
+rna1p is the Schizosaccharomyces pombe ortholog of the mammalian GTPase-activating protein (GAP) of Ran. Both proteins are essential for nuclear transport. Here, we report the crystal structure of rna1p at 2.66 A resolution. It contains 11 leucine-rich repeats that adopt the nonglobular shape of a crescent, bearing no resemblance to RhoGAP or RasGAP. The invariant residues of RanGAP form a contiguous surface, strongly indicating the Ran-binding interface. Alanine mutations identify Arg-74 as a critical residue for GTP hydrolysis. In contrast to RasGAP and RhoGAP, Arg-74 could be substituted by lysine and contributed significantly to the binding of Ran. Therefore, we suggest a GAP mechanism for rna1p, which constitutes a variation of the arginine finger mechanism found for Ras GAP and RhoGAP.
 ==About this Structure==
-YRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA].
+YRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Becker, J.]]
 [[Category: Drell, T.]]
-[[Category: Hillig, R.C.]]
+[[Category: Hillig, R C.]]
 [[Category: Renault, L.]]
-[[Category: Vetter, I.R.]]
+[[Category: Vetter, I R.]]
 [[Category: Wittinghofer, A.]]
 [[Category: gap]]
@@ Line 30: / Line 30: @@
 [[Category: twinning]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:00:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:24 2008''