1yyo: Difference between revisions
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New page: left|200px<br /><applet load="1yyo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyo, resolution 2.90Å" /> '''Crystal structure of... |
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[[Image:1yyo.gif|left|200px]]<br /><applet load="1yyo" size=" | [[Image:1yyo.gif|left|200px]]<br /><applet load="1yyo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1yyo, resolution 2.90Å" /> | caption="1yyo, resolution 2.90Å" /> | ||
'''Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with double-stranded RNA at 2.9-Angstrom Resolution'''<br /> | '''Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with double-stranded RNA at 2.9-Angstrom Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene | Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates. | ||
==About this Structure== | ==About this Structure== | ||
1YYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http:// | 1YYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Austin, B | [[Category: Austin, B P.]] | ||
[[Category: Court, D | [[Category: Court, D L.]] | ||
[[Category: Gan, J.]] | [[Category: Gan, J.]] | ||
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
[[Category: Tropea, J | [[Category: Tropea, J E.]] | ||
[[Category: Waugh, D | [[Category: Waugh, D S.]] | ||
[[Category: TRS]] | [[Category: TRS]] | ||
[[Category: double-stranded rna]] | [[Category: double-stranded rna]] | ||
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[[Category: rna interference]] | [[Category: rna interference]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:25 2008'' | ||
