1z5u: Difference between revisions

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[[Image:1z5u.gif|left|200px]]<br /><applet load="1z5u" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1z5u.gif|left|200px]]<br /><applet load="1z5u" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1z5u, resolution 2.3Å" />

'''Crystal structure of S. typhimurium AphA complexed with cyclic-AMP'''<br />

==Overview==

The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was, cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA, protein was purified to homogeneity. The protein crystallized in the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent, content of approximately 54%. The crystals are composed of biologically, active AphA molecules.

The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.

==About this Structure==

1Z5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG and CMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA].

1Z5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA].

==Reference==

Line 15:

[[Category: Single protein]]

[[Category: Kumar, V.]]

[[Category: Makde, R.D.]]

[[Category: Makde, R D.]]

[[Category: CMP]]

[[Category: MG]]

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[[Category: metalloenzyme]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:15:~~25 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:19 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1z5u.gif|left|200px]]<br /><applet load="1z5u" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1z5u.gif|left|200px]]<br /><applet load="1z5u" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1z5u, resolution 2.3&Aring;" />
 '''Crystal structure of S. typhimurium AphA complexed with cyclic-AMP'''<br />
 ==Overview==
-The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was, cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA, protein was purified to homogeneity. The protein crystallized in the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent, content of approximately 54%. The crystals are composed of biologically, active AphA molecules.
+The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
 ==About this Structure==
-Z5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG and CMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA].
+Z5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Kumar, V.]]
-[[Category: Makde, R.D.]]
+[[Category: Makde, R D.]]
 [[Category: CMP]]
 [[Category: MG]]
@@ Line 22: / Line 22: @@
 [[Category: metalloenzyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:15:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:19 2008''