1zla: Difference between revisions

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[[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1zla, resolution 2.9Å" />

'''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core'''<br />

==Overview==

Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear, antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We, find that N-terminal LANA docks onto chromosomes by binding nucleosomes, through the folded region of histones H2A-H2B. The same LANA residues were, required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B;, chromatin binding was rescued after assembly of nucleosomes containing, H2A-H2B. We also describe the 2.9-angstrom crystal structure of a, nucleosome complexed with the first 23 LANA amino acids. The LANA peptide, forms a hairpin that interacts exclusively with an acidic H2A-H2B region, that is implicated in the formation of higher order chromatin structure., Our findings present a paradigm for how nucleosomes may serve as binding, platforms for viral and cellular proteins and reveal a previously unknown, mechanism for KSHV latency.

Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.

==About this Structure==

1ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA].

1ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA].

==Reference==

Line 15:

[[Category: Protein complex]]

[[Category: Xenopus laevis]]

[[Category: Barbera, A.J.]]

[[Category: Barbera, A J.]]

[[Category: Chodaparambil, J.V.]]

[[Category: Chodaparambil, J V.]]

[[Category: Kaye, K.M.]]

[[Category: Kaye, K M.]]

[[Category: Luger, K.]]

[[Category: chromatin]]

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[[Category: protein/protein interaction]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1zla, resolution 2.9&Aring;" />
 '''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core'''<br />
 ==Overview==
-Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear, antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We, find that N-terminal LANA docks onto chromosomes by binding nucleosomes, through the folded region of histones H2A-H2B. The same LANA residues were, required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B;, chromatin binding was rescued after assembly of nucleosomes containing, H2A-H2B. We also describe the 2.9-angstrom crystal structure of a, nucleosome complexed with the first 23 LANA amino acids. The LANA peptide, forms a hairpin that interacts exclusively with an acidic H2A-H2B region, that is implicated in the formation of higher order chromatin structure., Our findings present a paradigm for how nucleosomes may serve as binding, platforms for viral and cellular proteins and reveal a previously unknown, mechanism for KSHV latency.
+Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.
 ==About this Structure==
-ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA].
+ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Protein complex]]
 [[Category: Xenopus laevis]]
-[[Category: Barbera, A.J.]]
+[[Category: Barbera, A J.]]
-[[Category: Chodaparambil, J.V.]]
+[[Category: Chodaparambil, J V.]]
-[[Category: Kaye, K.M.]]
+[[Category: Kaye, K M.]]
 [[Category: Luger, K.]]
 [[Category: chromatin]]
@@ Line 25: / Line 25: @@
 [[Category: protein/protein interaction]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:31:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:40 2008''