2a02: Difference between revisions

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[[Image:2a02.gif|left|200px]]<br /><applet load="2a02" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2a02.gif|left|200px]]<br /><applet load="2a02" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2a02" />

'''Solution NMR Structure of the Periplasmic Signaling Domain of the Outer Membrane Iron Transporter PupA from Pseudomonas putida.'''<br />

==Overview==

Transcription of the ferric citrate import system is regulated by ferric, citrate binding to the outer membrane transporter FecA. A signal, indicating transporter occupancy is relayed across the outer membrane to, energy-transducing and regulatory proteins embedded in the cytoplasmic, membrane. Because transcriptional activation is not coupled to ferric, citrate import, an allosteric mechanism underlies this complex signaling, mechanism. Using evolution-based statistical analysis we have identified a, sparse but structurally connected network of residues that links distant, functional sites in FecA. Functional analyses of these positions confirm, their involvement in the mechanism that regulates transcriptional, activation in response to ferric citrate binding at the cell surface. This, mechanism appears to be conserved and provides the structural basis for, the allosteric signaling of TonB-dependent transporters.

Transcription of the ferric citrate import system is regulated by ferric citrate binding to the outer membrane transporter FecA. A signal indicating transporter occupancy is relayed across the outer membrane to energy-transducing and regulatory proteins embedded in the cytoplasmic membrane. Because transcriptional activation is not coupled to ferric citrate import, an allosteric mechanism underlies this complex signaling mechanism. Using evolution-based statistical analysis we have identified a sparse but structurally connected network of residues that links distant functional sites in FecA. Functional analyses of these positions confirm their involvement in the mechanism that regulates transcriptional activation in response to ferric citrate binding at the cell surface. This mechanism appears to be conserved and provides the structural basis for the allosteric signaling of TonB-dependent transporters.

==About this Structure==

2A02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA].

2A02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA].

==Reference==

Line 13:

[[Category: Pseudomonas putida]]

[[Category: Single protein]]

[[Category: Amezcua, C.A.]]

[[Category: Amezcua, C A.]]

[[Category: Chelliah, Y.]]

[[Category: Deisenhofer, J.]]

[[Category: Ferguson, A.D.]]

[[Category: Ferguson, A D.]]

[[Category: Rosen, M.K.]]

[[Category: Rosen, M K.]]

[[Category: membrane protein]]

[[Category: metal transport]]

[[Category: protein nmr]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:50:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:19 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2a02.gif|left|200px]]<br /><applet load="2a02" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2a02.gif|left|200px]]<br /><applet load="2a02" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2a02" />
 '''Solution NMR Structure of the Periplasmic Signaling Domain of the Outer Membrane Iron Transporter PupA from Pseudomonas putida.'''<br />
 ==Overview==
-Transcription of the ferric citrate import system is regulated by ferric, citrate binding to the outer membrane transporter FecA. A signal, indicating transporter occupancy is relayed across the outer membrane to, energy-transducing and regulatory proteins embedded in the cytoplasmic, membrane. Because transcriptional activation is not coupled to ferric, citrate import, an allosteric mechanism underlies this complex signaling, mechanism. Using evolution-based statistical analysis we have identified a, sparse but structurally connected network of residues that links distant, functional sites in FecA. Functional analyses of these positions confirm, their involvement in the mechanism that regulates transcriptional, activation in response to ferric citrate binding at the cell surface. This, mechanism appears to be conserved and provides the structural basis for, the allosteric signaling of TonB-dependent transporters.
+Transcription of the ferric citrate import system is regulated by ferric citrate binding to the outer membrane transporter FecA. A signal indicating transporter occupancy is relayed across the outer membrane to energy-transducing and regulatory proteins embedded in the cytoplasmic membrane. Because transcriptional activation is not coupled to ferric citrate import, an allosteric mechanism underlies this complex signaling mechanism. Using evolution-based statistical analysis we have identified a sparse but structurally connected network of residues that links distant functional sites in FecA. Functional analyses of these positions confirm their involvement in the mechanism that regulates transcriptional activation in response to ferric citrate binding at the cell surface. This mechanism appears to be conserved and provides the structural basis for the allosteric signaling of TonB-dependent transporters.
 ==About this Structure==
-A02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA].
+A02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Pseudomonas putida]]
 [[Category: Single protein]]
-[[Category: Amezcua, C.A.]]
+[[Category: Amezcua, C A.]]
 [[Category: Chelliah, Y.]]
 [[Category: Deisenhofer, J.]]
-[[Category: Ferguson, A.D.]]
+[[Category: Ferguson, A D.]]
-[[Category: Rosen, M.K.]]
+[[Category: Rosen, M K.]]
 [[Category: membrane protein]]
 [[Category: metal transport]]
 [[Category: protein nmr]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:50:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:19 2008''