2a2u: Difference between revisions

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[[Image:2a2u.gif|left|200px]]<br /><applet load="2a2u" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2a2u.gif|left|200px]]<br /><applet load="2a2u" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2a2u, resolution 2.50Å" />

'''THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.'''<br />

==Overview==

Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult, male rats. The structure of a monoclinic crystal form of A2U was reported, in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The, structures of an orthorhombic crystal form of A2U at 2. 5 A resolution, (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between, A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor =, 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of, chemicals which cause a male rat-specific renal carcinogenesis called, hyaline-droplet nephropathy. The rate-determining step in the development, of this disorder is the binding of the toxin to A2U. Comparison of the, cavities in A2U and in the corresponding mouse urinary protein (MUP), reveal that the former is tailor-made for small oval hydrophobic ligands, such as DLO. The cavity in MUP is more shallow and elongated and cannot, easily accommodate such ligands.

Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult male rats. The structure of a monoclinic crystal form of A2U was reported in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The structures of an orthorhombic crystal form of A2U at 2. 5 A resolution (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor = 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of chemicals which cause a male rat-specific renal carcinogenesis called hyaline-droplet nephropathy. The rate-determining step in the development of this disorder is the binding of the toxin to A2U. Comparison of the cavities in A2U and in the corresponding mouse urinary protein (MUP) reveal that the former is tailor-made for small oval hydrophobic ligands such as DLO. The cavity in MUP is more shallow and elongated and cannot easily accommodate such ligands.

==About this Structure==

2A2U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2A2U OCA].

2A2U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2U OCA].

==Reference==

Line 13:

[[Category: Rattus norvegicus]]

[[Category: Single protein]]

[[Category: Chaudhuri, B.N.]]

[[Category: Chaudhuri, B N.]]

[[Category: Jones, T.A.]]

[[Category: Jones, T A.]]

[[Category: Kleywegt, G.J.]]

[[Category: Kleywegt, G J.]]

[[Category: a2u-globulin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:53:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2a2u.gif|left|200px]]<br /><applet load="2a2u" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2a2u.gif|left|200px]]<br /><applet load="2a2u" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2a2u, resolution 2.50&Aring;" />
 '''THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.'''<br />
 ==Overview==
-Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult, male rats. The structure of a monoclinic crystal form of A2U was reported, in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The, structures of an orthorhombic crystal form of A2U at 2. 5 A resolution, (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between, A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor =, 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of, chemicals which cause a male rat-specific renal carcinogenesis called, hyaline-droplet nephropathy. The rate-determining step in the development, of this disorder is the binding of the toxin to A2U. Comparison of the, cavities in A2U and in the corresponding mouse urinary protein (MUP), reveal that the former is tailor-made for small oval hydrophobic ligands, such as DLO. The cavity in MUP is more shallow and elongated and cannot, easily accommodate such ligands.
+Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult male rats. The structure of a monoclinic crystal form of A2U was reported in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The structures of an orthorhombic crystal form of A2U at 2. 5 A resolution (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor = 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of chemicals which cause a male rat-specific renal carcinogenesis called hyaline-droplet nephropathy. The rate-determining step in the development of this disorder is the binding of the toxin to A2U. Comparison of the cavities in A2U and in the corresponding mouse urinary protein (MUP) reveal that the former is tailor-made for small oval hydrophobic ligands such as DLO. The cavity in MUP is more shallow and elongated and cannot easily accommodate such ligands.
 ==About this Structure==
-A2U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A2U OCA].
+A2U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2U OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Rattus norvegicus]]
 [[Category: Single protein]]
-[[Category: Chaudhuri, B.N.]]
+[[Category: Chaudhuri, B N.]]
-[[Category: Jones, T.A.]]
+[[Category: Jones, T A.]]
-[[Category: Kleywegt, G.J.]]
+[[Category: Kleywegt, G J.]]
 [[Category: a2u-globulin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:53:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:02 2008''