2a8f: Difference between revisions

Line 1:

[[Image:2a8f.gif|left|200px]]<br /><applet load="2a8f" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2a8f.gif|left|200px]]<br /><applet load="2a8f" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2a8f, resolution 1.35Å" />

'''beta-cinnamomin after sterol removal'''<br />

==Overview==

The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was, determined in complex with ergosterol at 1.1 A resolution., beta-CIN/ergosterol complex crystallized in the monoclinic space group, P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and, beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand, extraction with chloroform followed by crystallographic analysis yielded a, 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the, characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The, necrotic effect of beta-CIN on tobacco was also shown to remain unchanged., Theoretical docking studies of the triterpene lupeol to beta-CIN provided, an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.

The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.

==About this Structure==

2A8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA].

2A8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Archer, M.]]

[[Category: Baptista, R.P.]]

[[Category: Baptista, R P.]]

[[Category: Carrondo, M.A.]]

[[Category: Carrondo, M A.]]

[[Category: Cravador, A.]]

[[Category: Enguita, F.J.]]

[[Category: Enguita, F J.]]

[[Category: Martel, P.]]

[[Category: Melo, E.P.]]

[[Category: Melo, E P.]]

[[Category: Miranda, S.]]

[[Category: Rodrigues, M.L.]]

[[Category: Rodrigues, M L.]]

[[Category: Sousa, N.]]

[[Category: Thomaz, M.]]

Line 29:

[[Category: sterol carrier protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:59:~~10 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:39 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2a8f.gif|left|200px]]<br /><applet load="2a8f" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2a8f.gif|left|200px]]<br /><applet load="2a8f" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2a8f, resolution 1.35&Aring;" />
 '''beta-cinnamomin after sterol removal'''<br />
 ==Overview==
-The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was, determined in complex with ergosterol at 1.1 A resolution., beta-CIN/ergosterol complex crystallized in the monoclinic space group, P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and, beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand, extraction with chloroform followed by crystallographic analysis yielded a, 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the, characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The, necrotic effect of beta-CIN on tobacco was also shown to remain unchanged., Theoretical docking studies of the triterpene lupeol to beta-CIN provided, an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
+The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
 ==About this Structure==
-A8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA].
+A8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Archer, M.]]
-[[Category: Baptista, R.P.]]
+[[Category: Baptista, R P.]]
-[[Category: Carrondo, M.A.]]
+[[Category: Carrondo, M A.]]
 [[Category: Cravador, A.]]
-[[Category: Enguita, F.J.]]
+[[Category: Enguita, F J.]]
 [[Category: Martel, P.]]
-[[Category: Melo, E.P.]]
+[[Category: Melo, E P.]]
 [[Category: Miranda, S.]]
-[[Category: Rodrigues, M.L.]]
+[[Category: Rodrigues, M L.]]
 [[Category: Sousa, N.]]
 [[Category: Thomaz, M.]]
@@ Line 29: / Line 29: @@
 [[Category: sterol carrier protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:59:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:39 2008''