2agl: Difference between revisions
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New page: left|200px<br /><applet load="2agl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2agl, resolution 1.400Å" /> '''Crystal structure o... |
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[[Image:2agl.gif|left|200px]]<br /><applet load="2agl" size=" | [[Image:2agl.gif|left|200px]]<br /><applet load="2agl" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2agl, resolution 1.400Å" /> | caption="2agl, resolution 1.400Å" /> | ||
'''Crystal structure of the phenylhydrazine adduct of aromatic amine dehydrogenase from Alcaligenes faecalis'''<br /> | '''Crystal structure of the phenylhydrazine adduct of aromatic amine dehydrogenase from Alcaligenes faecalis'''<br /> | ||
==Overview== | ==Overview== | ||
We present an atomic-level description of the reaction chemistry of an | We present an atomic-level description of the reaction chemistry of an enzyme-catalyzed reaction dominated by proton tunneling. By solving structures of reaction intermediates at near-atomic resolution, we have identified the reaction pathway for tryptamine oxidation by aromatic amine dehydrogenase. Combining experiment and computer simulation, we show proton transfer occurs predominantly to oxygen O2 of Asp(128)beta in a reaction dominated by tunneling over approximately 0.6 angstroms. The role of long-range coupled motions in promoting tunneling is controversial. We show that, in this enzyme system, tunneling is promoted by a short-range motion modulating proton-acceptor distance and no long-range coupled motion is required. | ||
==About this Structure== | ==About this Structure== | ||
2AGL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with PHZ as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase Aralkylamine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.4 1.4.99.4] Full crystallographic information is available from [http:// | 2AGL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=PHZ:'>PHZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase Aralkylamine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.4 1.4.99.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Basran, J.]] | [[Category: Basran, J.]] | ||
[[Category: Hothi, P.]] | [[Category: Hothi, P.]] | ||
[[Category: Johannissen, L | [[Category: Johannissen, L O.]] | ||
[[Category: Leys, D.]] | [[Category: Leys, D.]] | ||
[[Category: Masgrau, L.]] | [[Category: Masgrau, L.]] | ||
[[Category: Mulholland, A | [[Category: Mulholland, A J.]] | ||
[[Category: Ranaghan, K | [[Category: Ranaghan, K E.]] | ||
[[Category: Roujeinikova, A.]] | [[Category: Roujeinikova, A.]] | ||
[[Category: Scrutton, N | [[Category: Scrutton, N S.]] | ||
[[Category: Sutcliffe, M | [[Category: Sutcliffe, M J.]] | ||
[[Category: PHZ]] | [[Category: PHZ]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:12 2008'' | ||
