2aho: Difference between revisions

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[[Image:2aho.gif|left|200px]]<br /><applet load="2aho" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2aho.gif|left|200px]]<br /><applet load="2aho" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2aho, resolution 3.0Å" />

'''Structure of the archaeal initiation factor eIF2 alpha-gamma heterodimer from Sulfolobus solfataricus complexed with GDPNP'''<br />

==Overview==

Eukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric, proteins (alphabetagamma) supplying the small subunit of the ribosome with, methionylated initiator tRNA. This study reports the crystallographic, structure of an aIF2alphagamma heterodimer from Sulfolobus solfataricus, bound to Gpp(NH)p-Mg(2+). aIF2gamma is in a closed conformation with the G, domain packed on domains II and III. The C-terminal domain of aIF2alpha, interacts with domain II of aIF2gamma. Conformations of the two switch, regions involved in GTP binding are similar to those encountered in an, EF1A:GTP:Phe-tRNA(Phe) complex. Comparison with the EF1A structure, suggests that only the gamma subunit of the aIF2alphagamma heterodimer, contacts tRNA. Because the alpha subunit markedly reinforces the affinity, of tRNA for the gamma subunit, a contribution of the alpha subunit to the, switch movements observed in the gamma structure is considered.

Eukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric proteins (alphabetagamma) supplying the small subunit of the ribosome with methionylated initiator tRNA. This study reports the crystallographic structure of an aIF2alphagamma heterodimer from Sulfolobus solfataricus bound to Gpp(NH)p-Mg(2+). aIF2gamma is in a closed conformation with the G domain packed on domains II and III. The C-terminal domain of aIF2alpha interacts with domain II of aIF2gamma. Conformations of the two switch regions involved in GTP binding are similar to those encountered in an EF1A:GTP:Phe-tRNA(Phe) complex. Comparison with the EF1A structure suggests that only the gamma subunit of the aIF2alphagamma heterodimer contacts tRNA. Because the alpha subunit markedly reinforces the affinity of tRNA for the gamma subunit, a contribution of the alpha subunit to the switch movements observed in the gamma structure is considered.

==About this Structure==

2AHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with MG, ZN and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2AHO OCA].

2AHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHO OCA].

==Reference==

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[[Category: initiation of translation]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:07:~~56 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:31 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2aho.gif|left|200px]]<br /><applet load="2aho" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2aho.gif|left|200px]]<br /><applet load="2aho" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2aho, resolution 3.0&Aring;" />
 '''Structure of the archaeal initiation factor eIF2 alpha-gamma heterodimer from Sulfolobus solfataricus complexed with GDPNP'''<br />
 ==Overview==
-Eukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric, proteins (alphabetagamma) supplying the small subunit of the ribosome with, methionylated initiator tRNA. This study reports the crystallographic, structure of an aIF2alphagamma heterodimer from Sulfolobus solfataricus, bound to Gpp(NH)p-Mg(2+). aIF2gamma is in a closed conformation with the G, domain packed on domains II and III. The C-terminal domain of aIF2alpha, interacts with domain II of aIF2gamma. Conformations of the two switch, regions involved in GTP binding are similar to those encountered in an, EF1A:GTP:Phe-tRNA(Phe) complex. Comparison with the EF1A structure, suggests that only the gamma subunit of the aIF2alphagamma heterodimer, contacts tRNA. Because the alpha subunit markedly reinforces the affinity, of tRNA for the gamma subunit, a contribution of the alpha subunit to the, switch movements observed in the gamma structure is considered.
+Eukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric proteins (alphabetagamma) supplying the small subunit of the ribosome with methionylated initiator tRNA. This study reports the crystallographic structure of an aIF2alphagamma heterodimer from Sulfolobus solfataricus bound to Gpp(NH)p-Mg(2+). aIF2gamma is in a closed conformation with the G domain packed on domains II and III. The C-terminal domain of aIF2alpha interacts with domain II of aIF2gamma. Conformations of the two switch regions involved in GTP binding are similar to those encountered in an EF1A:GTP:Phe-tRNA(Phe) complex. Comparison with the EF1A structure suggests that only the gamma subunit of the aIF2alphagamma heterodimer contacts tRNA. Because the alpha subunit markedly reinforces the affinity of tRNA for the gamma subunit, a contribution of the alpha subunit to the switch movements observed in the gamma structure is considered.
 ==About this Structure==
-AHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with MG, ZN and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AHO OCA].
+AHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHO OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: initiation of translation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:07:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:31 2008''