2aka: Difference between revisions
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New page: left|200px<br /><applet load="2aka" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aka, resolution 1.90Å" /> '''Structure of the nuc... |
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[[Image:2aka.gif|left|200px]]<br /><applet load="2aka" size=" | [[Image:2aka.gif|left|200px]]<br /><applet load="2aka" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2aka, resolution 1.90Å" /> | caption="2aka, resolution 1.90Å" /> | ||
'''Structure of the nucleotide-free myosin II motor domain from Dictyostelium discoideum fused to the GTPase domain of dynamin 1 from Rattus norvegicus'''<br /> | '''Structure of the nucleotide-free myosin II motor domain from Dictyostelium discoideum fused to the GTPase domain of dynamin 1 from Rattus norvegicus'''<br /> | ||
==Overview== | ==Overview== | ||
Here, we present the 1.9-A crystal structure of the nucleotide-free GTPase | Here, we present the 1.9-A crystal structure of the nucleotide-free GTPase domain of dynamin 1 from Rattus norvegicus. The structure corresponds to an extended form of the canonical GTPase fold observed in Ras proteins. Both nucleotide-binding switch motifs are well resolved, adopting conformations that closely resemble a GTP-bound state not previously observed for nucleotide-free GTPases. Two highly conserved arginines, Arg-66 and Arg-67, greatly restrict the mobility of switch I and are ideally positioned to relay information about the nucleotide state to other parts of the protein. Our results support a model in which switch I residue Arg-59 gates GTP binding in an assembly-dependent manner and the GTPase effector domain functions as an assembly-dependent GTPase activating protein in the fashion of RGS-type GAPs. | ||
==About this Structure== | ==About this Structure== | ||
2AKA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Active as [http://en.wikipedia.org/wiki/Dynamin_GTPase Dynamin GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.5 3.6.5.5] Full crystallographic information is available from [http:// | 2AKA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Active as [http://en.wikipedia.org/wiki/Dynamin_GTPase Dynamin GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.5 3.6.5.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Becker, A.]] | [[Category: Becker, A.]] | ||
[[Category: Eschenburg, S.]] | [[Category: Eschenburg, S.]] | ||
[[Category: Kull, F | [[Category: Kull, F J.]] | ||
[[Category: Leonard, M.]] | [[Category: Leonard, M.]] | ||
[[Category: Manstein, D | [[Category: Manstein, D J.]] | ||
[[Category: Reubold, T | [[Category: Reubold, T F.]] | ||
[[Category: Schmid, S | [[Category: Schmid, S L.]] | ||
[[Category: Vallee, R | [[Category: Vallee, R B.]] | ||
[[Category: dynamin]] | [[Category: dynamin]] | ||
[[Category: fusion protein]] | [[Category: fusion protein]] | ||
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[[Category: myosin]] | [[Category: myosin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:18 2008'' | ||
