2alx: Difference between revisions
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New page: left|200px<br /><applet load="2alx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2alx, resolution 2.600Å" /> '''Ribonucleotide Redu... |
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[[Image:2alx.gif|left|200px]]<br /><applet load="2alx" size=" | [[Image:2alx.gif|left|200px]]<br /><applet load="2alx" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2alx, resolution 2.600Å" /> | caption="2alx, resolution 2.600Å" /> | ||
'''Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22'''<br /> | '''Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22'''<br /> | ||
==Overview== | ==Overview== | ||
A new crystal form of wild-type ribonucleotide reductase R2 from | A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms. | ||
==About this Structure== | ==About this Structure== | ||
2ALX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and HG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http:// | 2ALX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Ribonucleoside-diphosphate reductase]] | [[Category: Ribonucleoside-diphosphate reductase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Jr., J | [[Category: Jr., J M.Bollinger.]] | ||
[[Category: Rosenzweig, A | [[Category: Rosenzweig, A C.]] | ||
[[Category: Saleh, L.]] | [[Category: Saleh, L.]] | ||
[[Category: Sommerhalter, M.]] | [[Category: Sommerhalter, M.]] | ||
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[[Category: ribonucleotide reductase r2]] | [[Category: ribonucleotide reductase r2]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:41 2008'' | ||
