2apj: Difference between revisions

Line 1:

[[Image:2apj.gif|left|200px]]<br /><applet load="2apj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2apj.gif|left|200px]]<br /><applet load="2apj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2apj, resolution 1.600Å" />

'''X-Ray Structure of Protein from Arabidopsis Thaliana AT4G34215 at 1.6 Angstrom Resolution'''<br />

==Overview==

The crystal structure of the At4g34215 protein of Arabidopsis thaliana was, determined by molecular replacement and refined to an R factor of 14.6%, (R(free) = 18.3%) at 1.6 Angstroms resolution. The crystal structure, confirms that At4g34215 belongs to the SGNH-hydrolase superfamily of, enzymes. The catalytic triad of the enzyme comprises residues Ser31, His238 and Asp235. In this structure the catalytic serine residue was, found to be covalently modified, possibly by phenylmethylsulfonyl, fluoride. The structure also reveals a previously undescribed variation, within the active site. The conserved asparagine from block III, which, provides a hydrogen bond for an oxyanion hole in the SGNH-hydrolase, superfamily enzymes, is missing in At4g34215 and is functionally replaced, by Gln30 from block I. This residue is positioned in a catalytically, competent conformation by nearby residues, including Gln159, Gly160 and, Glu161, which are fully conserved in the carbohydrate esterase family 6, enzymes.

The crystal structure of the At4g34215 protein of Arabidopsis thaliana was determined by molecular replacement and refined to an R factor of 14.6% (R(free) = 18.3%) at 1.6 Angstroms resolution. The crystal structure confirms that At4g34215 belongs to the SGNH-hydrolase superfamily of enzymes. The catalytic triad of the enzyme comprises residues Ser31, His238 and Asp235. In this structure the catalytic serine residue was found to be covalently modified, possibly by phenylmethylsulfonyl fluoride. The structure also reveals a previously undescribed variation within the active site. The conserved asparagine from block III, which provides a hydrogen bond for an oxyanion hole in the SGNH-hydrolase superfamily enzymes, is missing in At4g34215 and is functionally replaced by Gln30 from block I. This residue is positioned in a catalytically competent conformation by nearby residues, including Gln159, Gly160 and Glu161, which are fully conserved in the carbohydrate esterase family 6 enzymes.

==About this Structure==

2APJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure ~~superseeds~~ the now removed PDB entry 2AEA. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2APJ OCA].

2APJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry 2AEA. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2APJ OCA].

==Reference==

Line 13:

[[Category: Arabidopsis thaliana]]

[[Category: Single protein]]

[[Category: Allard, S.T.]]

[[Category: Allard, S T.]]

[[Category: Bingman, C.A.]]

[[Category: Bingman, C A.]]

[[Category: Bitto, E.]]

[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]

[[Category: CESG, Center for Eukaryotic Structural Genomics.]]

[[Category: Jr., G.N.Phillips.]]

[[Category: Jr., G N.Phillips.]]

[[Category: Mccoy, J.G.]]

[[Category: Mccoy, J G.]]

[[Category: Wesenberg, G.E.]]

[[Category: Wesenberg, G E.]]

[[Category: at4g34215]]

[[Category: carbohydrate esterase family 6]]

Line 30:

[[Category: structural genomics]]

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@@ Line 1: / Line 1: @@
-[[Image:2apj.gif|left|200px]]<br /><applet load="2apj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2apj.gif|left|200px]]<br /><applet load="2apj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2apj, resolution 1.600&Aring;" />
 '''X-Ray Structure of Protein from Arabidopsis Thaliana AT4G34215 at 1.6 Angstrom Resolution'''<br />
 ==Overview==
-The crystal structure of the At4g34215 protein of Arabidopsis thaliana was, determined by molecular replacement and refined to an R factor of 14.6%, (R(free) = 18.3%) at 1.6 Angstroms resolution. The crystal structure, confirms that At4g34215 belongs to the SGNH-hydrolase superfamily of, enzymes. The catalytic triad of the enzyme comprises residues Ser31, His238 and Asp235. In this structure the catalytic serine residue was, found to be covalently modified, possibly by phenylmethylsulfonyl, fluoride. The structure also reveals a previously undescribed variation, within the active site. The conserved asparagine from block III, which, provides a hydrogen bond for an oxyanion hole in the SGNH-hydrolase, superfamily enzymes, is missing in At4g34215 and is functionally replaced, by Gln30 from block I. This residue is positioned in a catalytically, competent conformation by nearby residues, including Gln159, Gly160 and, Glu161, which are fully conserved in the carbohydrate esterase family 6, enzymes.
+The crystal structure of the At4g34215 protein of Arabidopsis thaliana was determined by molecular replacement and refined to an R factor of 14.6% (R(free) = 18.3%) at 1.6 Angstroms resolution. The crystal structure confirms that At4g34215 belongs to the SGNH-hydrolase superfamily of enzymes. The catalytic triad of the enzyme comprises residues Ser31, His238 and Asp235. In this structure the catalytic serine residue was found to be covalently modified, possibly by phenylmethylsulfonyl fluoride. The structure also reveals a previously undescribed variation within the active site. The conserved asparagine from block III, which provides a hydrogen bond for an oxyanion hole in the SGNH-hydrolase superfamily enzymes, is missing in At4g34215 and is functionally replaced by Gln30 from block I. This residue is positioned in a catalytically competent conformation by nearby residues, including Gln159, Gly160 and Glu161, which are fully conserved in the carbohydrate esterase family 6 enzymes.
 ==About this Structure==
-APJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure superseeds the now removed PDB entry 2AEA. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2APJ OCA].
+APJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry 2AEA. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2APJ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Arabidopsis thaliana]]
 [[Category: Single protein]]
-[[Category: Allard, S.T.]]
+[[Category: Allard, S T.]]
-[[Category: Bingman, C.A.]]
+[[Category: Bingman, C A.]]
 [[Category: Bitto, E.]]
-[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]
+[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
-[[Category: Jr., G.N.Phillips.]]
+[[Category: Jr., G N.Phillips.]]
-[[Category: Mccoy, J.G.]]
+[[Category: Mccoy, J G.]]
-[[Category: Wesenberg, G.E.]]
+[[Category: Wesenberg, G E.]]
 [[Category: at4g34215]]
 [[Category: carbohydrate esterase family 6]]
@@ Line 30: / Line 30: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:16:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:29:36 2008''