2aro: Difference between revisions
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New page: left|200px<br /><applet load="2aro" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aro, resolution 2.10Å" /> '''Crystal Structure Of... |
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[[Image:2aro.gif|left|200px]]<br /><applet load="2aro" size=" | [[Image:2aro.gif|left|200px]]<br /><applet load="2aro" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2aro, resolution 2.10Å" /> | caption="2aro, resolution 2.10Å" /> | ||
'''Crystal Structure Of The Native Histone Octamer To 2.1 Angstrom Resolution, Crystalised In The Presence Of S-Nitrosoglutathione'''<br /> | '''Crystal Structure Of The Native Histone Octamer To 2.1 Angstrom Resolution, Crystalised In The Presence Of S-Nitrosoglutathione'''<br /> | ||
==Overview== | ==Overview== | ||
A H3 dimer band is produced when purified native histone octamers are run | A H3 dimer band is produced when purified native histone octamers are run on an SDS-PAGE gel in a beta-mercaptoethanol-free environment. To investigate this, native histone octamer crystals, derived from chicken erythrocytes, and of structure (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A'), were grown in 2 M KCl, 1.35 M potassium phosphates and 250-350 microM of the oxidising agent S-nitrosoglutathione, pH 6.9. X-ray diffraction data were acquired to 2.10 A resolution, yielding a structure with an Rwork value of 18.6% and an Rfree of 22.5%. The space group is P6(5), the asymmetric unit of which contains one complete octamer. Compared to the 1.90 A resolution, unoxidised native histone octamer structure, the crystals show a reduction of 2.5% in the c-axis of the unit cell, and free-energy calculations reveal that the H3-H3' dimer interface in the latter has become thermodynamically stable, in contrast to the former. Although the inter-sulphur distance of the two H3 cysteines in the oxidised native histone octamer has reduced to 6 A from the 7 A of the unoxidised form, analysis of the hydrogen bonds that constitute the (H4-H3)-(H3'-H4') tetramer indicates that the formation of a disulphide bond in the H3-H3' dimer interface is incompatible with stable tetramer formation. The biochemical and biophysical evidence, taken as a whole, is indicative of crystals that have a stable H3-H3' dimer interface, possibly extending to the interface within an isolated H3-H3' dimer, observed in SDS-PAGE gels. | ||
==About this Structure== | ==About this Structure== | ||
2ARO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with PO4 and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2ARO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ARO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Baldwin, J | [[Category: Baldwin, J P.]] | ||
[[Category: Lambert, S | [[Category: Lambert, S J.]] | ||
[[Category: Nicholson, J | [[Category: Nicholson, J M.]] | ||
[[Category: Reynolds, C | [[Category: Reynolds, C D.]] | ||
[[Category: Sodngam, S.]] | [[Category: Sodngam, S.]] | ||
[[Category: Wood, C | [[Category: Wood, C M.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: oxidation]] | [[Category: oxidation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:17 2008'' | ||
