2atf: Difference between revisions
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New page: left|200px<br /><applet load="2atf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2atf, resolution 1.75Å" /> '''X-RAY STRUCTURE OF c... |
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[[Image:2atf.gif|left|200px]]<br /><applet load="2atf" size=" | [[Image:2atf.gif|left|200px]]<br /><applet load="2atf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2atf, resolution 1.75Å" /> | caption="2atf, resolution 1.75Å" /> | ||
'''X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056'''<br /> | '''X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056'''<br /> | ||
==Overview== | ==Overview== | ||
Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to | Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% identical to that of a human homolog. The structure reveals that CDO adopts the typical beta-barrel fold of the cupin superfamily. The NE2 atoms of His-86, -88, and -140 provide the metal binding site. The structure further revealed a covalent linkage between the side chains of Cys-93 and Tyr-157, the cysteine of which is conserved only in eukaryotic proteins. Metal analysis showed that the recombinant enzyme contained a mixture of iron, nickel, and zinc, with increased iron content associated with increased catalytic activity. Details of the predicted active site are used to present and discuss a plausible mechanism of action for the enzyme. | ||
==About this Structure== | ==About this Structure== | ||
2ATF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NI and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cysteine_dioxygenase Cysteine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.20 1.13.11.20] Full crystallographic information is available from [http:// | 2ATF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cysteine_dioxygenase Cysteine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.20 1.13.11.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allard, S | [[Category: Allard, S T.M.]] | ||
[[Category: Bingman, C | [[Category: Bingman, C A.]] | ||
[[Category: Bitto, E.]] | [[Category: Bitto, E.]] | ||
[[Category: CESG, Center | [[Category: CESG, Center for Eukaryotic Structural Genomics.]] | ||
[[Category: Jr., G | [[Category: Jr., G N.Phillips.]] | ||
[[Category: Mccoy, J | [[Category: Mccoy, J G.]] | ||
[[Category: Wesenberg, G | [[Category: Wesenberg, G E.]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: NI]] | [[Category: NI]] | ||
| Line 27: | Line 27: | ||
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: cupin family]] | [[Category: cupin family]] | ||
[[Category: mm | [[Category: mm 241056]] | ||
[[Category: pfam05995 | [[Category: pfam05995 2 cdo_i]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:49 2008'' | ||
