2az2: Difference between revisions

Line 1:

[[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2az2, resolution 2.60Å" />

'''Flock House virus B2-dsRNA Complex (P4122)'''<br />

==Overview==

As a counter-defense against antiviral RNA silencing during infection, the, insect Flock House virus (FHV) expresses the silencing suppressor protein, B2. Biochemical experiments show that B2 binds to double-stranded RNA, (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer, in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix, bundle that binds to one face of an A-form RNA duplex independently of, sequence. These results suggest that B2 blocks both cleavage of the FHV, genome by Dicer and incorporation of FHV small interfering RNAs into the, RNA-induced silencing complex.

As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.

==About this Structure==

2AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2AZ2 OCA].

2AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZ2 OCA].

==Reference==

Line 13:

[[Category: Flock house virus]]

[[Category: Single protein]]

[[Category: Chao, J.A.]]

[[Category: Chao, J A.]]

[[Category: Chapados, B.R.]]

[[Category: Chapados, B R.]]

[[Category: Debler, E.W.]]

[[Category: Debler, E W.]]

[[Category: Lee, J.H.]]

[[Category: Lee, J H.]]

[[Category: Schneemann, A.]]

[[Category: Williamson, J.R.]]

[[Category: Williamson, J R.]]

[[Category: four-helix bundle]]

[[Category: protein-rna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:28:33 ~~2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:33 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2az2, resolution 2.60&Aring;" />
 '''Flock House virus B2-dsRNA Complex (P4122)'''<br />
 ==Overview==
-As a counter-defense against antiviral RNA silencing during infection, the, insect Flock House virus (FHV) expresses the silencing suppressor protein, B2. Biochemical experiments show that B2 binds to double-stranded RNA, (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer, in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix, bundle that binds to one face of an A-form RNA duplex independently of, sequence. These results suggest that B2 blocks both cleavage of the FHV, genome by Dicer and incorporation of FHV small interfering RNAs into the, RNA-induced silencing complex.
+As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.
 ==About this Structure==
-AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AZ2 OCA].
+AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZ2 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Flock house virus]]
 [[Category: Single protein]]
-[[Category: Chao, J.A.]]
+[[Category: Chao, J A.]]
-[[Category: Chapados, B.R.]]
+[[Category: Chapados, B R.]]
-[[Category: Debler, E.W.]]
+[[Category: Debler, E W.]]
-[[Category: Lee, J.H.]]
+[[Category: Lee, J H.]]
 [[Category: Schneemann, A.]]
-[[Category: Williamson, J.R.]]
+[[Category: Williamson, J R.]]
 [[Category: four-helix bundle]]
 [[Category: protein-rna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:28:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:33 2008''