2b2d: Difference between revisions

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[[Image:2b2d.gif|left|200px]]<br /><applet load="2b2d" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2b2d.gif|left|200px]]<br /><applet load="2b2d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2b2d, resolution 2.9Å" />

'''RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid'''<br />

==Overview==

Sequence-specific interactions between RNA stem-loops and coat protein, (CP) subunits play vital roles in the life cycles of the RNA, bacteriophages, e.g., by allowing translational repression of their, replicase cistrons and tagging their own RNA genomes for encapsidation., The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of, their cognate translational operators, even in the presence of closely, related operators from other phages in vivo. Discrete mutations within the, MS2 CP have been shown to relax this discrimination in vitro. We have, determined the structures of eight complexes between such mutants and both, MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with, previously determined in vivo repression data, the structures enable us to, propose the molecular basis for the discrimination mechanism.

Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

==About this Structure==

2B2D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA].

2B2D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA].

==Reference==

Line 13:

[[Category: Enterobacterio phage ms2]]

[[Category: Single protein]]

[[Category: Adams, C.J.]]

[[Category: Adams, C J.]]

[[Category: Baron, A.J.]]

[[Category: Baron, A J.]]

[[Category: Grahn, E.]]

[[Category: Helgstrand, C.]]

[[Category: Horn, W.T.]]

[[Category: Horn, W T.]]

[[Category: Lago, H.]]

[[Category: Liljas, L.]]

[[Category: Peabody, D.S.]]

[[Category: Peabody, D S.]]

[[Category: Phillips, S.E.V.]]

[[Category: Phillips, S E.V.]]

[[Category: Stockley, P.G.]]

[[Category: Stockley, P G.]]

[[Category: Stonehouse, N.J.]]

[[Category: Stonehouse, N J.]]

[[Category: Tars, K.]]

[[Category: capsid]]

Line 31:

[[Category: virus/viral]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:31:~~58 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2b2d.gif|left|200px]]<br /><applet load="2b2d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2b2d.gif|left|200px]]<br /><applet load="2b2d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2b2d, resolution 2.9&Aring;" />
 '''RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid'''<br />
 ==Overview==
-Sequence-specific interactions between RNA stem-loops and coat protein, (CP) subunits play vital roles in the life cycles of the RNA, bacteriophages, e.g., by allowing translational repression of their, replicase cistrons and tagging their own RNA genomes for encapsidation., The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of, their cognate translational operators, even in the presence of closely, related operators from other phages in vivo. Discrete mutations within the, MS2 CP have been shown to relax this discrimination in vitro. We have, determined the structures of eight complexes between such mutants and both, MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with, previously determined in vivo repression data, the structures enable us to, propose the molecular basis for the discrimination mechanism.
+Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
 ==About this Structure==
-B2D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA].
+B2D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Enterobacterio phage ms2]]
 [[Category: Single protein]]
-[[Category: Adams, C.J.]]
+[[Category: Adams, C J.]]
-[[Category: Baron, A.J.]]
+[[Category: Baron, A J.]]
 [[Category: Grahn, E.]]
 [[Category: Helgstrand, C.]]
-[[Category: Horn, W.T.]]
+[[Category: Horn, W T.]]
 [[Category: Lago, H.]]
 [[Category: Liljas, L.]]
-[[Category: Peabody, D.S.]]
+[[Category: Peabody, D S.]]
-[[Category: Phillips, S.E.V.]]
+[[Category: Phillips, S E.V.]]
-[[Category: Stockley, P.G.]]
+[[Category: Stockley, P G.]]
-[[Category: Stonehouse, N.J.]]
+[[Category: Stonehouse, N J.]]
 [[Category: Tars, K.]]
 [[Category: capsid]]
@@ Line 31: / Line 31: @@
 [[Category: virus/viral]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:31:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:26 2008''