2b61: Difference between revisions
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New page: left|200px<br /><applet load="2b61" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b61, resolution 1.650Å" /> '''Crystal Structure o... |
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[[Image:2b61.gif|left|200px]]<br /><applet load="2b61" size=" | [[Image:2b61.gif|left|200px]]<br /><applet load="2b61" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2b61, resolution 1.650Å" /> | caption="2b61, resolution 1.650Å" /> | ||
'''Crystal Structure of Homoserine Transacetylase'''<br /> | '''Crystal Structure of Homoserine Transacetylase'''<br /> | ||
==Overview== | ==Overview== | ||
Homoserine transacetylase catalyzes one of the required steps in the | Homoserine transacetylase catalyzes one of the required steps in the biosynthesis of methionine in fungi and several bacteria. We have determined the crystal structure of homoserine transacetylase from Haemophilus influenzae to a resolution of 1.65 A. The structure identifies this enzyme to be a member of the alpha/beta-hydrolase structural superfamily. The active site of the enzyme is located near the end of a deep tunnel formed by the juxtaposition of two domains and incorporates a catalytic triad involving Ser143, His337, and Asp304. A structural basis is given for the observed double displacement kinetic mechanism of homoserine transacetylase. Furthermore, the properties of the tunnel provide a rationale for how homoserine transacetylase catalyzes a transferase reaction vs hydrolysis, despite extensive similarity in active site architecture to hydrolytic enzymes. | ||
==About this Structure== | ==About this Structure== | ||
2B61 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Homoserine_O-acetyltransferase Homoserine O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.31 2.3.1.31] Full crystallographic information is available from [http:// | 2B61 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Homoserine_O-acetyltransferase Homoserine O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.31 2.3.1.31] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B61 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homoserine O-acetyltransferase]] | [[Category: Homoserine O-acetyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Berghuis, A | [[Category: Berghuis, A M.]] | ||
[[Category: Korczynska, M.]] | [[Category: Korczynska, M.]] | ||
[[Category: Mirza, I | [[Category: Mirza, I A.]] | ||
[[Category: Nazi, I.]] | [[Category: Nazi, I.]] | ||
[[Category: Wright, G | [[Category: Wright, G D.]] | ||
[[Category: acyl-enzyme]] | [[Category: acyl-enzyme]] | ||
[[Category: alpha-beta hydrolase fold]] | [[Category: alpha-beta hydrolase fold]] | ||
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[[Category: structure-function studies]] | [[Category: structure-function studies]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:32 2008'' | ||
