2b7m: Difference between revisions

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[[Image:2b7m.gif|left|200px]]<br /><applet load="2b7m" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2b7m.gif|left|200px]]<br /><applet load="2b7m" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2b7m, resolution 3.50Å" />

'''Crystal Structure of the S. cerevisiae Exocyst Component Exo70p'''<br />

==Overview==

The exocyst is an evolutionarily conserved multiprotein complex required, for the targeting and docking of post-Golgi vesicles to the plasma, membrane. Through its interactions with a variety of proteins, including, small GTPases, the exocyst is thought to integrate signals from the cell, and signal that vesicles arriving at the plasma membrane are ready for, fusion. Here we describe the three-dimensional crystal structure of one of, the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at, 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent, manner with an equilibrium dissociation constant of approximately 70, microM. Exo70p is an extended rod approximately 155 angstroms in length, composed principally of alpha helices, and is a novel fold. The structure, provides a first view of the Exo70 protein family and provides a framework, to study the molecular function of this exocyst component.

The exocyst is an evolutionarily conserved multiprotein complex required for the targeting and docking of post-Golgi vesicles to the plasma membrane. Through its interactions with a variety of proteins, including small GTPases, the exocyst is thought to integrate signals from the cell and signal that vesicles arriving at the plasma membrane are ready for fusion. Here we describe the three-dimensional crystal structure of one of the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent manner with an equilibrium dissociation constant of approximately 70 microM. Exo70p is an extended rod approximately 155 angstroms in length composed principally of alpha helices, and is a novel fold. The structure provides a first view of the Exo70 protein family and provides a framework to study the molecular function of this exocyst component.

==About this Structure==

2B7M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2B7M OCA].

2B7M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7M OCA].

==Reference==

Line 13:

[[Category: Saccharomyces cerevisiae]]

[[Category: Single protein]]

[[Category: Hamburger, A.E.]]

[[Category: Hamburger, A E.]]

[[Category: Hamburger, Z.A.]]

[[Category: Hamburger, Z A.]]

[[Category: Weis, W.I.]]

[[Category: Weis, W I.]]

[[Category: West, A.P.]]

[[Category: West, A P.]]

[[Category: exocyst]]

[[Category: exocytosis]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:37:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:59 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2b7m.gif|left|200px]]<br /><applet load="2b7m" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2b7m.gif|left|200px]]<br /><applet load="2b7m" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2b7m, resolution 3.50&Aring;" />
 '''Crystal Structure of the S. cerevisiae Exocyst Component Exo70p'''<br />
 ==Overview==
-The exocyst is an evolutionarily conserved multiprotein complex required, for the targeting and docking of post-Golgi vesicles to the plasma, membrane. Through its interactions with a variety of proteins, including, small GTPases, the exocyst is thought to integrate signals from the cell, and signal that vesicles arriving at the plasma membrane are ready for, fusion. Here we describe the three-dimensional crystal structure of one of, the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at, 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent, manner with an equilibrium dissociation constant of approximately 70, microM. Exo70p is an extended rod approximately 155 angstroms in length, composed principally of alpha helices, and is a novel fold. The structure, provides a first view of the Exo70 protein family and provides a framework, to study the molecular function of this exocyst component.
+The exocyst is an evolutionarily conserved multiprotein complex required for the targeting and docking of post-Golgi vesicles to the plasma membrane. Through its interactions with a variety of proteins, including small GTPases, the exocyst is thought to integrate signals from the cell and signal that vesicles arriving at the plasma membrane are ready for fusion. Here we describe the three-dimensional crystal structure of one of the components of the exocyst, Exo70p, from Saccharomyces cerevisiae at 3.5A resolution. Exo70p binds the small GTPase Rho3p in a GTP-dependent manner with an equilibrium dissociation constant of approximately 70 microM. Exo70p is an extended rod approximately 155 angstroms in length composed principally of alpha helices, and is a novel fold. The structure provides a first view of the Exo70 protein family and provides a framework to study the molecular function of this exocyst component.
 ==About this Structure==
-B7M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B7M OCA].
+B7M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7M OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Hamburger, A.E.]]
+[[Category: Hamburger, A E.]]
-[[Category: Hamburger, Z.A.]]
+[[Category: Hamburger, Z A.]]
-[[Category: Weis, W.I.]]
+[[Category: Weis, W I.]]
-[[Category: West, A.P.]]
+[[Category: West, A P.]]
 [[Category: exocyst]]
 [[Category: exocytosis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:37:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:59 2008''