2bjv: Difference between revisions

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[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2bjv, resolution 1.70Å" />

'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''<br />

==Overview==

Activators of bacterial sigma54-RNA polymerase holoenzyme are, mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis, to activate transcription. We have determined by cryogenic electron, microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition, state analog in complex with its basal factor, sigma54. By fitting the, crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we, identified two loops involved in binding sigma54. Comparing, enhancer-binding structures in different nucleotide states and mutational, analysis led us to propose nucleotide-dependent conformational changes, that free the loops for association with sigma54.

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

==About this Structure==

2BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].

2BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].

==Reference==

Line 16:

[[Category: Bordes, P.]]

[[Category: Buck, M.]]

[[Category: Keetch, C.A.]]

[[Category: Keetch, C A.]]

[[Category: Niwa, H.]]

[[Category: Rappas, M.]]

[[Category: Robinson, C.V.]]

[[Category: Robinson, C V.]]

[[Category: Schumacher, J.]]

[[Category: Wigneshweraraj, S.]]

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[[Category: transcription regulation]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:33 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2bjv, resolution 1.70&Aring;" />
 '''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''<br />
 ==Overview==
-Activators of bacterial sigma54-RNA polymerase holoenzyme are, mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis, to activate transcription. We have determined by cryogenic electron, microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition, state analog in complex with its basal factor, sigma54. By fitting the, crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we, identified two loops involved in binding sigma54. Comparing, enhancer-binding structures in different nucleotide states and mutational, analysis led us to propose nucleotide-dependent conformational changes, that free the loops for association with sigma54.
+Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
 ==About this Structure==
-BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
+BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Bordes, P.]]
 [[Category: Buck, M.]]
-[[Category: Keetch, C.A.]]
+[[Category: Keetch, C A.]]
 [[Category: Niwa, H.]]
 [[Category: Rappas, M.]]
-[[Category: Robinson, C.V.]]
+[[Category: Robinson, C V.]]
 [[Category: Schumacher, J.]]
 [[Category: Wigneshweraraj, S.]]
@@ Line 33: / Line 33: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:48:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:33 2008''