2bn8: Difference between revisions

Line 1:

[[Image:2bn8.jpg|left|200px]]<br /><applet load="2bn8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2bn8.jpg|left|200px]]<br /><applet load="2bn8" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2bn8" />

'''SOLUTION STRUCTURE AND INTERACTIONS OF THE E.COLI CELL DIVISION ACTIVATOR PROTEIN CEDA'''<br />

==Overview==

CedA is a protein that is postulated to be involved in the regulation of, cell division in Escherichia coli and related organisms; however, little, biological data about its possible mode of action are available. Here we, present a three-dimensional structure of this protein as determined by NMR, spectroscopy. The protein is made up of four antiparallel beta-strands, an, alpha-helix, and a large unstructured stretch of residues at the, N-terminus. It shows structural similarity to a family of DNA-binding, proteins which interact with dsDNA via a three-stranded beta-sheet, suggesting that CedA may be a DNA-binding protein. The putative binding, surface of CedA is predominantly positively charged with a number of basic, residues surrounding a groove largely dominated by aromatic residues. NMR, chemical shift perturbations and gel-shift experiments performed with CedA, confirm that the protein binds dsDNA, and its interaction is mediated, primarily via the beta-sheet.

CedA is a protein that is postulated to be involved in the regulation of cell division in Escherichia coli and related organisms; however, little biological data about its possible mode of action are available. Here we present a three-dimensional structure of this protein as determined by NMR spectroscopy. The protein is made up of four antiparallel beta-strands, an alpha-helix, and a large unstructured stretch of residues at the N-terminus. It shows structural similarity to a family of DNA-binding proteins which interact with dsDNA via a three-stranded beta-sheet, suggesting that CedA may be a DNA-binding protein. The putative binding surface of CedA is predominantly positively charged with a number of basic residues surrounding a groove largely dominated by aromatic residues. NMR chemical shift perturbations and gel-shift experiments performed with CedA confirm that the protein binds dsDNA, and its interaction is mediated primarily via the beta-sheet.

==About this Structure==

2BN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA].

2BN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Chen, H.A.]]

[[Category: Chen, H A.]]

[[Category: Huyton, T.]]

[[Category: Matthews, S.]]

Line 22:

[[Category: cell division activator protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:50:~~29 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:37 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2bn8.jpg|left|200px]]<br /><applet load="2bn8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bn8.jpg|left|200px]]<br /><applet load="2bn8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2bn8" />
 '''SOLUTION STRUCTURE AND INTERACTIONS OF THE E.COLI CELL DIVISION ACTIVATOR PROTEIN CEDA'''<br />
 ==Overview==
-CedA is a protein that is postulated to be involved in the regulation of, cell division in Escherichia coli and related organisms; however, little, biological data about its possible mode of action are available. Here we, present a three-dimensional structure of this protein as determined by NMR, spectroscopy. The protein is made up of four antiparallel beta-strands, an, alpha-helix, and a large unstructured stretch of residues at the, N-terminus. It shows structural similarity to a family of DNA-binding, proteins which interact with dsDNA via a three-stranded beta-sheet, suggesting that CedA may be a DNA-binding protein. The putative binding, surface of CedA is predominantly positively charged with a number of basic, residues surrounding a groove largely dominated by aromatic residues. NMR, chemical shift perturbations and gel-shift experiments performed with CedA, confirm that the protein binds dsDNA, and its interaction is mediated, primarily via the beta-sheet.
+CedA is a protein that is postulated to be involved in the regulation of cell division in Escherichia coli and related organisms; however, little biological data about its possible mode of action are available. Here we present a three-dimensional structure of this protein as determined by NMR spectroscopy. The protein is made up of four antiparallel beta-strands, an alpha-helix, and a large unstructured stretch of residues at the N-terminus. It shows structural similarity to a family of DNA-binding proteins which interact with dsDNA via a three-stranded beta-sheet, suggesting that CedA may be a DNA-binding protein. The putative binding surface of CedA is predominantly positively charged with a number of basic residues surrounding a groove largely dominated by aromatic residues. NMR chemical shift perturbations and gel-shift experiments performed with CedA confirm that the protein binds dsDNA, and its interaction is mediated primarily via the beta-sheet.
 ==About this Structure==
-BN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA].
+BN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Chen, H.A.]]
+[[Category: Chen, H A.]]
 [[Category: Huyton, T.]]
 [[Category: Matthews, S.]]
@@ Line 22: / Line 22: @@
 [[Category: cell division activator protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:50:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:37 2008''