2boe: Difference between revisions
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New page: left|200px<br /><applet load="2boe" size="450" color="white" frame="true" align="right" spinBox="true" caption="2boe, resolution 1.15Å" /> '''CATALYTIC DOMAIN OF ... |
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[[Image:2boe.gif|left|200px]]<br /><applet load="2boe" size=" | [[Image:2boe.gif|left|200px]]<br /><applet load="2boe" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2boe, resolution 1.15Å" /> | caption="2boe, resolution 1.15Å" /> | ||
'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA'''<br /> | '''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA'''<br /> | ||
==Overview== | ==Overview== | ||
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution. | ||
==About this Structure== | ==About this Structure== | ||
2BOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http:// | 2BOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Driguez, H.]] | [[Category: Driguez, H.]] | ||
[[Category: Dultz, E.]] | [[Category: Dultz, E.]] | ||
[[Category: Irwin, D | [[Category: Irwin, D C.]] | ||
[[Category: Jones, T | [[Category: Jones, T A.]] | ||
[[Category: Larsson, A | [[Category: Larsson, A M.]] | ||
[[Category: Roos, A.]] | [[Category: Roos, A.]] | ||
[[Category: Wilson, D | [[Category: Wilson, D B.]] | ||
[[Category: carbohydrate metabolism]] | [[Category: carbohydrate metabolism]] | ||
[[Category: cellulose degradation]] | [[Category: cellulose degradation]] | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:56 2008'' | ||
