2ciu: Difference between revisions

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[[Image:2ciu.jpg|left|200px]]<br /><applet load="2ciu" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2ciu.jpg|left|200px]]<br /><applet load="2ciu" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2ciu, resolution 1.6Å" />

'''STRUCTURE OF THE IMS DOMAIN OF THE MITOCHONDRIAL IMPORT PROTEIN TIM21 FROM S. CEREVISIAE'''<br />

==Overview==

Proteins destined for the mitochondrial matrix are imported by the, translocase of the outer membrane--the TOM complex--and the presequence, translocase of the inner membrane--the TIM23 complex. At present, there is, no structural information on components of the presequence translocase., Tim21, a subunit of the presequence translocase consisting of a membrane, anchor and a carboxy-terminal domain exposed to the intermembrane space, directly connects the TOM and TIM23 complexes by binding to the, intermembrane space domain of the Tom22 receptor. We crystallized the, binding domain of Tim21 of Saccharomyces cerevisiae and determined its, structure at 1.6 A resolution. The Tim21 structure represents a new, alpha/beta-mixed protein fold with two alpha-helices flanked by an, extended eight-stranded beta-sheet. We also identified a core sequence of, Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid, residues of Tom22 are important for binding to Tim21. Here we suggest a, mechanism for the TOM-TIM interaction.

Proteins destined for the mitochondrial matrix are imported by the translocase of the outer membrane--the TOM complex--and the presequence translocase of the inner membrane--the TIM23 complex. At present, there is no structural information on components of the presequence translocase. Tim21, a subunit of the presequence translocase consisting of a membrane anchor and a carboxy-terminal domain exposed to the intermembrane space, directly connects the TOM and TIM23 complexes by binding to the intermembrane space domain of the Tom22 receptor. We crystallized the binding domain of Tim21 of Saccharomyces cerevisiae and determined its structure at 1.6 A resolution. The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction.

==About this Structure==

2CIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA].

2CIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA].

==Reference==

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[[Category: transport]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 09:08:~~15 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:06 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2ciu.jpg|left|200px]]<br /><applet load="2ciu" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ciu.jpg|left|200px]]<br /><applet load="2ciu" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2ciu, resolution 1.6&Aring;" />
 '''STRUCTURE OF THE IMS DOMAIN OF THE MITOCHONDRIAL IMPORT PROTEIN TIM21 FROM S. CEREVISIAE'''<br />
 ==Overview==
-Proteins destined for the mitochondrial matrix are imported by the, translocase of the outer membrane--the TOM complex--and the presequence, translocase of the inner membrane--the TIM23 complex. At present, there is, no structural information on components of the presequence translocase., Tim21, a subunit of the presequence translocase consisting of a membrane, anchor and a carboxy-terminal domain exposed to the intermembrane space, directly connects the TOM and TIM23 complexes by binding to the, intermembrane space domain of the Tom22 receptor. We crystallized the, binding domain of Tim21 of Saccharomyces cerevisiae and determined its, structure at 1.6 A resolution. The Tim21 structure represents a new, alpha/beta-mixed protein fold with two alpha-helices flanked by an, extended eight-stranded beta-sheet. We also identified a core sequence of, Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid, residues of Tom22 are important for binding to Tim21. Here we suggest a, mechanism for the TOM-TIM interaction.
+Proteins destined for the mitochondrial matrix are imported by the translocase of the outer membrane--the TOM complex--and the presequence translocase of the inner membrane--the TIM23 complex. At present, there is no structural information on components of the presequence translocase. Tim21, a subunit of the presequence translocase consisting of a membrane anchor and a carboxy-terminal domain exposed to the intermembrane space, directly connects the TOM and TIM23 complexes by binding to the intermembrane space domain of the Tom22 receptor. We crystallized the binding domain of Tim21 of Saccharomyces cerevisiae and determined its structure at 1.6 A resolution. The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction.
 ==About this Structure==
-CIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA].
+CIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA].
 ==Reference==
@@ Line 27: / Line 27: @@
 [[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:08:15 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:06 2008''