2cvq: Difference between revisions

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[[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2cvq, resolution 2.08Å" />

'''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''<br />

==Overview==

For better understanding of the coenzyme specificity in NAD-dependent MDH, (tMDH) from Thermus flavus AT-62, we determined the crystal structures of, tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure, is almost the same as that of the tMDH-NADH complex. However, NADP(H), binds to tMDH in the reverse orientation, where adenine occupies the, position near the catalytic center and nicotinamide is positioned at the, adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+), inhibited tMDH at high concentrations. This has provided the first, evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.

For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.

==About this Structure==

2CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with NAP and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA].

2CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=NAP:'>NAP</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA].

==Reference==

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[[Category: nad-dependent-mdh nadp+ complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 09:17:~~26 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:50 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2cvq, resolution 2.08&Aring;" />
 '''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''<br />
 ==Overview==
-For better understanding of the coenzyme specificity in NAD-dependent MDH, (tMDH) from Thermus flavus AT-62, we determined the crystal structures of, tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure, is almost the same as that of the tMDH-NADH complex. However, NADP(H), binds to tMDH in the reverse orientation, where adenine occupies the, position near the catalytic center and nicotinamide is positioned at the, adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+), inhibited tMDH at high concentrations. This has provided the first, evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.
+For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.
 ==About this Structure==
-CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with NAP and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA].
+CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=NAP:'>NAP</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: nad-dependent-mdh nadp+ complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:17:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:50 2008''