2cw0: Difference between revisions
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New page: left|200px<br /><applet load="2cw0" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cw0, resolution 3.3Å" /> '''Crystal structure of ... |
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[[Image:2cw0.gif|left|200px]]<br /><applet load="2cw0" size=" | [[Image:2cw0.gif|left|200px]]<br /><applet load="2cw0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2cw0, resolution 3.3Å" /> | caption="2cw0, resolution 3.3Å" /> | ||
'''Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution'''<br /> | '''Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution'''<br /> | ||
==Overview== | ==Overview== | ||
We define the target, mechanism, and structural basis of inhibition of | We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents. | ||
==About this Structure== | ==About this Structure== | ||
2CW0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | 2CW0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Arnold, E.]] | [[Category: Arnold, E.]] | ||
[[Category: Birktoft, J | [[Category: Birktoft, J J.]] | ||
[[Category: Borukhov, S.]] | [[Category: Borukhov, S.]] | ||
[[Category: Dharia, C.]] | [[Category: Dharia, C.]] | ||
[[Category: Ebright, R | [[Category: Ebright, R H.]] | ||
[[Category: Hudson, B.]] | [[Category: Hudson, B.]] | ||
[[Category: Ismail, S.]] | [[Category: Ismail, S.]] | ||
[[Category: Jr., A | [[Category: Jr., A D.Clark.]] | ||
[[Category: Laptenko, O.]] | [[Category: Laptenko, O.]] | ||
[[Category: Lee, J.]] | [[Category: Lee, J.]] | ||
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[[Category: Mukhopadhyay, J.]] | [[Category: Mukhopadhyay, J.]] | ||
[[Category: Napoli, A.]] | [[Category: Napoli, A.]] | ||
[[Category: Sarafianos, S | [[Category: Sarafianos, S G.]] | ||
[[Category: Sineva, E.]] | [[Category: Sineva, E.]] | ||
[[Category: Tuske, S.]] | [[Category: Tuske, S.]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:59 2008'' | ||
