2dep: Difference between revisions

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[[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2dep, resolution 1.80Å" />

'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''<br />

==Overview==

Recombinant mature xylanase B from Clostridium stercorarium has been, prepared and crystallized by the sitting-drop vapour-diffusion method, using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable, crystal grew after incubation for ten weeks at 293 K. The crystal belonged, to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b =, 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A, resolution.

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.

==About this Structure==

2DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].

2DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].

==Reference==

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[[Category: xylan degradation]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 09:34:~~18 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2dep, resolution 1.80&Aring;" />
 '''Crystal Structure of xylanase B from Clostridium stercorarium F9'''<br />
 ==Overview==
-Recombinant mature xylanase B from Clostridium stercorarium has been, prepared and crystallized by the sitting-drop vapour-diffusion method, using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable, crystal grew after incubation for ten weeks at 293 K. The crystal belonged, to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b =, 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A, resolution.
+Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
 ==About this Structure==
-DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].
+DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].
 ==Reference==
@@ Line 25: / Line 25: @@
 [[Category: xylan degradation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:34:18 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:02 2008''