2dkb: Difference between revisions
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New page: left|200px<br /><applet load="2dkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dkb, resolution 2.1Å" /> '''DIALKYLGLYCINE DECARB... |
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[[Image:2dkb.jpg|left|200px]]<br /><applet load="2dkb" size=" | [[Image:2dkb.jpg|left|200px]]<br /><applet load="2dkb" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2dkb, resolution 2.1Å" /> | caption="2dkb, resolution 2.1Å" /> | ||
'''DIALKYLGLYCINE DECARBOXYLASE STRUCTURE: BIFUNCTIONAL ACTIVE SITE AND ALKALI METAL BINDING SITES'''<br /> | '''DIALKYLGLYCINE DECARBOXYLASE STRUCTURE: BIFUNCTIONAL ACTIVE SITE AND ALKALI METAL BINDING SITES'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of the bifunctional, pyridoxal phosphate-dependent enzyme | The structure of the bifunctional, pyridoxal phosphate-dependent enzyme dialkylglycine decarboxylase was determined to 2.1-angstrom resolution. Model building suggests that a single cleavage site catalyzes both decarboxylation and transamination by maximizing stereoelectronic advantages and providing electrostatic and general base catalysis. The enzyme contains two binding sites for alkali metal ions. One is located near the active site and accounts for the dependence of activity on potassium ions. The other is located at the carboxyl terminus of an alpha helix. These sites help show how proteins can specifically bind alkali metals and how these ions can exert functional effects. | ||
==About this Structure== | ==About this Structure== | ||
2DKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia] with NA, PLP and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2,2-dialkylglycine_decarboxylase_(pyruvate) 2,2-dialkylglycine decarboxylase (pyruvate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.64 4.1.1.64] Full crystallographic information is available from [http:// | 2DKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2,2-dialkylglycine_decarboxylase_(pyruvate) 2,2-dialkylglycine decarboxylase (pyruvate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.64 4.1.1.64] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DKB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hohenester, E.]] | [[Category: Hohenester, E.]] | ||
[[Category: Jansonius, J | [[Category: Jansonius, J N.]] | ||
[[Category: Toney, M | [[Category: Toney, M D.]] | ||
[[Category: MES]] | [[Category: MES]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: lyase(decarboxylase)]] | [[Category: lyase(decarboxylase)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:59:52 2008'' | ||
