2esw: Difference between revisions

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[[Image:2esw.gif|left|200px]]<br /><applet load="2esw" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2esw.gif|left|200px]]<br /><applet load="2esw" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2esw, resolution 2.01Å" />

'''Atomic structure of the N-terminal SH3 domain of mouse beta PIX,p21-activated kinase (PAK)-interacting exchange factor'''<br />

==Overview==

The mouse betaPIX-SH3 domain, residues 8-63 of P21-activated kinase, interacting exchange factor, has been characterized by X-ray diffraction., Crystals belonging to space group P3(2)21 diffracted to 2.0 A and the, structure was phased by the single-wavelength anomalous diffraction, method. The domain is a compact beta-barrel with an overall conformation, similar to the general SH3 structure. The X-ray structure shows mouse, betaPIX-SH3 domain binding the way in which the betaPIX characteristic, amino acids do so for an unconventional ligand binding surface. This, arrangement provides a rationale for the unusual ligand recognition motif, exhibited by mouse betaPIX-SH3 domain. Comparison with another SH3/peptide, complex shows that the recognition mode of the mouse betaPIX-SH3 domain, should be very similar to the RXXK ligand binding mode. The unique large, and planar hydrophobic pocket may contribute to the promiscuity of, betaPIX-SH3 domain resulting in its multiple biological functions.

The mouse betaPIX-SH3 domain, residues 8-63 of P21-activated kinase interacting exchange factor, has been characterized by X-ray diffraction. Crystals belonging to space group P3(2)21 diffracted to 2.0 A and the structure was phased by the single-wavelength anomalous diffraction method. The domain is a compact beta-barrel with an overall conformation similar to the general SH3 structure. The X-ray structure shows mouse betaPIX-SH3 domain binding the way in which the betaPIX characteristic amino acids do so for an unconventional ligand binding surface. This arrangement provides a rationale for the unusual ligand recognition motif exhibited by mouse betaPIX-SH3 domain. Comparison with another SH3/peptide complex shows that the recognition mode of the mouse betaPIX-SH3 domain should be very similar to the RXXK ligand binding mode. The unique large and planar hydrophobic pocket may contribute to the promiscuity of betaPIX-SH3 domain resulting in its multiple biological functions.

==About this Structure==

2ESW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with HG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2ESW OCA].

2ESW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ESW OCA].

==Reference==

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[[Category: sh3 domain]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:22 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2esw.gif|left|200px]]<br /><applet load="2esw" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2esw.gif|left|200px]]<br /><applet load="2esw" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2esw, resolution 2.01&Aring;" />
 '''Atomic structure of the N-terminal SH3 domain of mouse beta PIX,p21-activated kinase (PAK)-interacting exchange factor'''<br />
 ==Overview==
-The mouse betaPIX-SH3 domain, residues 8-63 of P21-activated kinase, interacting exchange factor, has been characterized by X-ray diffraction., Crystals belonging to space group P3(2)21 diffracted to 2.0 A and the, structure was phased by the single-wavelength anomalous diffraction, method. The domain is a compact beta-barrel with an overall conformation, similar to the general SH3 structure. The X-ray structure shows mouse, betaPIX-SH3 domain binding the way in which the betaPIX characteristic, amino acids do so for an unconventional ligand binding surface. This, arrangement provides a rationale for the unusual ligand recognition motif, exhibited by mouse betaPIX-SH3 domain. Comparison with another SH3/peptide, complex shows that the recognition mode of the mouse betaPIX-SH3 domain, should be very similar to the RXXK ligand binding mode. The unique large, and planar hydrophobic pocket may contribute to the promiscuity of, betaPIX-SH3 domain resulting in its multiple biological functions.
+The mouse betaPIX-SH3 domain, residues 8-63 of P21-activated kinase interacting exchange factor, has been characterized by X-ray diffraction. Crystals belonging to space group P3(2)21 diffracted to 2.0 A and the structure was phased by the single-wavelength anomalous diffraction method. The domain is a compact beta-barrel with an overall conformation similar to the general SH3 structure. The X-ray structure shows mouse betaPIX-SH3 domain binding the way in which the betaPIX characteristic amino acids do so for an unconventional ligand binding surface. This arrangement provides a rationale for the unusual ligand recognition motif exhibited by mouse betaPIX-SH3 domain. Comparison with another SH3/peptide complex shows that the recognition mode of the mouse betaPIX-SH3 domain should be very similar to the RXXK ligand binding mode. The unique large and planar hydrophobic pocket may contribute to the promiscuity of betaPIX-SH3 domain resulting in its multiple biological functions.
 ==About this Structure==
-ESW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with HG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ESW OCA].
+ESW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ESW OCA].
 ==Reference==
@@ Line 19: / Line 19: @@
 [[Category: sh3 domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:07:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:22 2008''